Summary
During stimulation of the anterior lobe of the cerebellum, postsynaptic potentials were recorded intracellularly from ipsilateral Deiters neurones of the cat. In the majority of examined cells, the inhibitory postsynapic potentials were induced with short latency; 1.06 msec on the average from lobule III or IV. The latency was longer (1.23 msec) when the lobule V was stimulated, while it was shorter (0.86 msec) from the juxtafastigial region. It follows that the IPSP was produced via a monosynaptic pathway at a conduction velocity of 15 to 20 m/sec. Recording of the extracellular field potentials and focal stimulation within and around Deiters' nucleus further indicated that the inhibitory impulses propagated out of the cerebellum along a remarkable bundle of fibres which terminated within Deiters' nucleus. These results are all explicable by assuming that the cerebellar Purkinje cells are inhibitory in nature and so produce IPSPs monosynaptically in Deiters neurones via the long corticofugal fibres. Monosynaptic EPSPs were also detected in some Deiters neurones. They are considered to be mediated by the other pathways formed of axon collaterals of the cerebellar afferents.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andersen, P., J.C. Eccles, and Y. Løyning: Location of postsynaptic inhibitory synapses of hippocampal pyramids. J. Neurophysiol. 27, 592–607 (1964).
— and P.E. Voorhoeve: Postsynaptic inhibition of cerebellar Purkinje cells. J. Neurophysiol. 27, 1138–1153 (1964).
Bremer, F.: Contribution a l'étude de la physiologie du cervelet. La fonction inhibitrice du paleo-cerebellum. Arch. internat. physiol. 19, 189–226 (1922).
Brodal, A., O. Pompeiano, and F. Walberg: The vestibular nuclei and their connexions. Springfield Ill.: C.C. Thomas 1962.
Brookhart, J.M.: The cerebellum. In Textbook of Physiology, Sect. I. Neurophysiology, II. 1245–1280 (1959).
Cajal, S. Ramón Y: Histologie du systeme nerveux de l'homme et des vertébrés. Paris 1909.
Denny-Bbown, D., J.C. Eccles, and E.G.T. Liddell: Observations on electrical stimulation of the cerebellar cortex. Proc. Roy. Soc., B, Lond. 104, 518–536 (1929).
deVito, R.V., A. Brusa and A. Arduini: Cerebellar and vestibular influences on Deitersian units. J. Neurophysiol. 19, 241–253 (1956).
Dow, R.S., and G. Moruzzi: The Physiology and Pathology of the Cerebellum. Mineapolis: Univ. Minnesota Press 1958.
Eager, R.P.: Efferent cortico-nuclear pathways in the cerebellum of the cat. J. Comp. Neurol. 120, 81–104 (1963).
Eccles, J.C.: The Physiology of Synapses. pp 38, 123, 173–188. Berlin-Göttingen-Heidelberg: Springer 1964.
—, P. Fatt and K. Koketsu: Cholinergic and inhibitory synapses in a pathway from motoraxon cellaterals to motoneurones. J. Physiol. 126, 524–562 (1954).
— and S. Landgren: Central pathway for direct inhibitory action on impulses in largest afferent nerve fibres to muscle. J. Neurophysiol. 19, 75–98 (1956).
— J.I. Hubbard and O. Oscarsson: Intracellular recording from cells of the ventral spinocerebellar tract. J. Physiol. 158, 486–516 (1961).
— E. Magni and W.D. Willis: Depolarization of central terminals of group I afferent fibres from muscle. J. Physiol. 160, 62–93 (1962).
— R. Llinás and K. Sasaki: Excitation of cerebellar Purkinje cells by the climbing fibres. Nature, Lond. 203, 245–246 (1964).
— R. Llinás and K. Sasaki The excitatory synaptic action of climbing fibres on the Purkinje cells of the cerebellum. J. Physiol. 182, 268–296 (1966).
Gernandt, E., and S. Gilman: Descending vestibular activity and its modulation by proprioceptive, cerebellar and reticular influences. Exp. Neurol. 1, 273–304 (1959).
Granit, R., and C.G. Phillips: Excitatory and inhibitory processes acting upon individual Purkinje cells of the cerebellum in cats. J. Physiol. 133, 520–547 (1956).
Holmqvist, B., A. Lundberg and O. Oscarsson: A supraspinal control system monosynaptically connected with an ascending spinal pathway. Arch. ital. Biol. 98, 402–422 (1960).
Hursh, L.B.: Conduction velocity and diameter of nerve fibres. Amer. J. Physiol. 127, 131–139 (1939).
Ito, M.: New electronic device for stimulating and recording potentials for a single ganglion cell. In Medical Electronics. pp. 84–85, ed by Smyth, C.N. London: Iliff. (1960).
—, and N. Kawai: IPSP-receptive field in the cerebellum for Deiters neurones. Proc. Jap. Acad. 40, 762–764 (1964a).
—, and M. Yoshida: The cerebellar-evoked monosynaptic inhibition of Deiters neurones. Experientia 20, 516–516 (1964b).
— K. Obata and R. Ochi: Initiation of IPSP in Deiters' and fastigial neurones associated with the activity of cerebellar Purkinje cells. Proc. Jap. Acad. 40, 765–768 (1964c).
— M. Yoshida and K. Obata: Monosynaptic inhibition of the intracerebellar nuclei induced from the cerebellar cortex. Experientia 20, 575–576 (1964d).
— T. Hongo, M. Yoshida, Y. Okada and K. Obata: Intracellularly recorded antidromic responses of Deiters neurones. Experientia 20, 295–296 (1964e).
— Antidromic and trans-synaptic activation of Deiters neurones induced from the spinal cord. Jap. J. Physiol. 14, 638–658 (1964f).
Ito, M., N. Kawai and M. Udo: The origin of cerebellar-induced inhibition and facilitation in the neurones of Deiters' and intracerebellar nuclei. XXIII Int. Congr. Physiol. Sci. Tokyo, 997 (1965).
— K. Obata and R. Ochi: The origin of cerebellar-induced inhibition of Deiters neurones. II. Temporal correlation between the trans-synaptic activation of Purkinje cells and the inhibition of Deiters neurones. Exp. Brain Res. 2, 350–364 (1966).
Jakob, A.: Das Kleinhirn. In Handbuch der Mikroskopischen Anatomie des Menschen. Berlin: P 780 Springer (1928).
Katz, B., and R. Miledi: Propagation of electric activity in motor nerve terminals Proc. Roy. Soc., B, Lond. 161, 443–482 (1965).
Larsell, O.: The cerebellum of the cat and the monkey. J. Comp. Neurol. 99, 135–200 (1953).
Lorente de Nó, R.: Vestibulo-ocular reflex arc. Arch. Neurol Psych. 30, 245–291 (1933).
Lowenthal, M., and V. Horsley: On the relations between the cerebellar and other centers (namely cerebral and spinal) with special reference to the action of antagonistic muscles. Proc. Roy. Soc., B. Lond. 61, 20–25 (1897).
Moruzzi, G.: Problems in Cerebellar Physiology. Springfield: Thomas 1950.
Pollock, L.J., and L. Davis: The reflex activities of a decerebrate animal. J. Comp. Neurol. 50, 377–411 (1930).
Pompeiano, O., and E. Cotti: Analisi microelectrodica delle propiezioni cerebellodeitersiane. Arch. Sci. biol. 43, 57–101 (1959). Quoted from Brodal et al. (1962).
Sherrington, C.S.: Double (antidromic) conduction in the central nervous system. Proc. Roy. Soc., B. Lond. 61, 243–246 (1897).
Szentágothai-Schimert, J.: Die Bedeutung des Faserkalibers und der Markscheidendicke im Zentralnervensystem. Zeit. Anat. Entwickl. 111, 201–223 (1941).
Walberg, F., and J. Jansen: Cerebellar corticovestibular fibres in the cat. Exp. Neurol. 3, 32–52 (1961).
Wall, P.D.: Excitability changes in afferent fibre terminations and their relation to slow potentials. J. Physiol. 142, 1–22 (1958).
Wilson, V.J., and P.R. Burgess: Disinhibition in the cat spinal cord. J. Neurophysiol. 25, 392–404 (1962).
Author information
Authors and Affiliations
Additional information
The authors wish to thank Prof. Sir John Eccles for his comments on the manuscript and for improving the English.
Rights and permissions
About this article
Cite this article
Ito, M., Yoshida, M. The origin of cerebellar-induced inhibition of Deiters neurones I. Monosynaptic initiation of the inhibitory postsynaptic potentials. Exp Brain Res 2, 330–349 (1966). https://doi.org/10.1007/BF00234779
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00234779