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Effect of oxygen-enriched aeration on regeneration of rice (Oryza sativa L.) cell culture

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Abstract

The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/ℓ, the efficiency of regeneration was highest at 12 mg/ℓ. In the oxygenenriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.

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Abbreviations

DO:

dissolved oxygen

2,4-D:

2,4-dichlorophenoxyacetic acid

MES:

2-(N-morpholino) ethanesulfonic acid

rpm:

revolution per minute

NAA:

1-naphthaleneacetic acid

vvm:

volume per volume per minute

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Author notes

  1. Akihiro Okamoto

    Present address: Nagoya Plant, Kirin Brewery Co. Ltd., Terano, Shinkawa-cho, Nishikasugai-gun, 452, Aichi, Japan

Authors and Affiliations

  1. First Research Center, Nursery Technology Inc., Kitsuregawa-machi, Shioya-gun, 329-14, Tochigi, Japan

    Akihiro Okamoto, Shinobu Kishine & Takayasu Hirosawa

  2. Third Research Center, Nursery Technology Inc., Kawasaki, 211, Kanagawa, Japan

    Atsuyuki Nakazono

Authors
  1. Akihiro Okamoto
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  2. Shinobu Kishine
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  3. Takayasu Hirosawa
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  4. Atsuyuki Nakazono
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Communicated by A. Komamine

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Okamoto, A., Kishine, S., Hirosawa, T. et al. Effect of oxygen-enriched aeration on regeneration of rice (Oryza sativa L.) cell culture. Plant Cell Reports 15, 731–736 (1996). https://doi.org/10.1007/BF00232217

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  • DOI: https://doi.org/10.1007/BF00232217

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