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Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures

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Summary

Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibited ADC activity, cellular putrescine content, DNA synthesis, and cell division. The effect was reversible by exogenous putrescine. Ornithine decarboxylase (ODC; EC 4.1.1.17) activity was always less than 10% of the ADC activity. Addition of DL α-difluoromethylornithine had no effect on ODC activity, cellular polyamine levels, DNA synthesis, and cell division within the first 24 h but by 48 to 72 h it did inhibit these activities. Methylglyoxal bis(guanyl-hydrazone) inhibited S-adenosylmethionine decarboxylase (EC 4.1.1.50) activity without affecting DNA synthesis and cell division.

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Abbreviations

ADC:

arginine decarboxylase

ODC:

ornithine decarboxylase

SAMDC:

S-adenosylmethionine decarboxylase

DFMA:

DL α-difluoro-methylarginine

DFMO:

DL α-difluoromethylornithine

MGBG:

methylglyoxal bis(guanylhydrazone)

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Authors and Affiliations

  1. USDA Forest Service, NEFES, P.O. Box 640, 03824, Durham, NH, USA

    R. Minocha & W. C. Shortle

  2. Department of Plant Biology, University of New Hampshire, 03824, Durham, NH, USA

    S. C. Minocha

  3. Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, 980, Sendai, Japan

    A. Komamine

Authors
  1. R. Minocha
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  2. S. C. Minocha
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  3. A. Komamine
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  4. W. C. Shortle
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Communicated by A. R. Gould

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Minocha, R., Minocha, S.C., Komamine, A. et al. Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures. Plant Cell Reports 10, 126–130 (1991). https://doi.org/10.1007/BF00232042

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  • DOI: https://doi.org/10.1007/BF00232042

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