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Production of theanine and other γ-glutamyl derivatives by Camellia sinensis cultured cells

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Abstract

The maximum theanine production by Camellia sinensis cultured cells was achieved by culturing in the modified MS medium containing 2 mg/l indole-3-butyric acid, 0.1 mg/l kinetin, 0 mM NH4NO3 and 39.6 mM KNO3 with 40 mM ethylamine hydrochloride or 20 mM ethylamine hydrochloride and 10 mM L-glutamic acid. Other primary amines, such as methylamine, n-butylamine, 2-hydroxyethylamine, 2cyanoethylamine, aniline, benzylamine and phenylethylamine, were also biotransformed to N5-alkyl-L-glutamine derivatives by C. sinensis cultured cells.

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Abbreviations

IBA:

indole-3-butyric acid

K:

kinetin

MS medium:

Murashige and Skoog's basal medium (1962)

NMR:

nuclear magnetic resonance

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Kitasato University, Minato-ku, 108, Tokyo, Japan

    Yutaka Orihara & Tsutomu Furuya

Authors
  1. Yutaka Orihara
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  2. Tsutomu Furuya
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Additional information

Communicated by A. R. Gould

Part 70 in the series “Studies on Plant Tissue Culture”. For Part 69 see Furuya et al. (1990).

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Orihara, Y., Furuya, T. Production of theanine and other γ-glutamyl derivatives by Camellia sinensis cultured cells. Plant Cell Reports 9, 65–68 (1990). https://doi.org/10.1007/BF00231550

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  • DOI: https://doi.org/10.1007/BF00231550

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