Summary
Seed protein profiles of nine diploid species (2n = 20), ten tetraploid accessions, two synthetic amphidiploids and two autotetraploids (2n = 40) were studied using SDS-polyacrylamide gel electrophoresis. While the general profiles suggested considerable homology among these taxa in spite of speciation and ploidy differences, appreciable genetic differences were present to support the existing genomic divisions and sub-divisions in the section Arachis. A high degree of relationship was indicated between the two diploid species (A. duranensis containing the A genome and A. batizocoi (ICG 8210) containing the B genome) and tetraploids A. monticola/ A. hypogaea (2n = 40) containing AABB genome. Similar relationships were recorded between the AABB synthetic amphidiploid and the profile obtained from the mixture of protein of A. duranensis and A. batizocoi, suggesting that these two diploid species were the donors of the A and B genome, respectively, to tetraploid A. monticola/A. hypogaea.
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References
Cherry JP (1975) Comparative studies of seed proteins and enzymes of species and collections of Arachis by gel electrophoresis. Peanut Sci 2:57–65
Klozova E, Svachulova J, Smartt J, Hadac E, Turkova V, Hadacova V (1983a) The comparison of seed protein pattern within the genus Arachis by polyacrylamide gel electrophoresis. Biol Plant 25:266–273
Klozova L, Turkova V, Smartt J, Pitterova K, Svachulova J (1983 b) Immunochemical characterization of seed protein of some species of the genus Arachis L. Biol Plant 25:201–208
Krishna TG, Mitra R (1988) The probable genome donors to Arachis hypogaea L. based on arachin seed storage protein. Euphytica 37:47–52
Ladizinsky G (1975) Seed protein electrophoresis of the wild and cultivated species of section Faba of Vicia. Euphytica 24:785–788
Ladizinsky G, Hymowitz T (1979) Seed protein electrophoresis in taxonomic and evolutionary studies. Theor Appl Genet 54:145–151
Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951), Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Margolaish E, Fitch WM (1968) Evolutionary variability of cytochrome C primary structure. Ann NY Acad Sci 151:359–381
McDaniel RG (1970) Electrophoretic characterization of protein in Hordeum. J Hered 61:243–247
Rao CR (1952) Advanced statistical methods in biometric research. John Wiley and Sons, New York
Singh AK (1988) Putative genome donors of Arachis hypogaea (Fabaceae), evidence from crosses with synthetic amphidiploids. Plant Syst Evol 160:143–151
Singh AK, Moss JP (1982) Utilization of wild relatives in genetic improvement of Arachis hypogaea L. 2. Chromosome complements of species of section Arachis. Theor Appl Genet 61:305–314
Singh AK, Moss JP (1984) Utilization of wild relatives in genetic improvement of Arachis hypogaea L. 5. Genome analysis in section Arachis and its implications in gene transfer. Theor Appl Genet 68:355–364
Smartt J, Gregory WC, Gregory MP (1978) The genome of Arachis hypogaea I. Cytogenetic studies of putative genome donors. Euphytica 27:665–675
Stalker HT, Dalmacio RD (1981) Chromosomes of Arachis species, Section Arachis. J Hered 72:403–408
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Communicated by A. R. Hallaver
Submitted as Journal Article No. 1114 by International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
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Singh, A.K., Sivaramakrishnan, S., Mengesha, M.H. et al. Phylogenetic relations in section Arachis based on seed protein profile. Theoret. Appl. Genetics 82, 593–597 (1991). https://doi.org/10.1007/BF00226795
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DOI: https://doi.org/10.1007/BF00226795