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Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences

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Abstract

A set of six cloned barley (Hordeum vulgare) repetitive DNA sequences was used for the analysis of phylogenetic relationships among 31 species (46 taxa) of the genus Hordeum, using molecular hybridization techniques. in situ hybridization experiments showed dispersed organization of the sequences over all chromosomes of H. vulgare and the wild barley species H. bulbosum, H. marinum and H. murinum. Southern blot hybridization revealed different levels of polymorphism among barley species and the RFLP data were used to generate a phylogenetic tree for the genus Hordeum. Our data are in a good agreement with the classification system which suggests the division of the genus into four major groups, containing the genomes I, X, Y, and H. However, our investigation also supports previous molecular studies of barley species where the unique position of H. bulbosum has been pointed out. In our experiments, H. bulbosum generally had hybridization patterns different from those of H. vulgare, although both carry the I genome. Based on our results we present a hypothesis concerning the possible origin and phylogeny of the polyploid barley species H. secalinum, H. depressum and the H. brachyantherum complex.

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Author notes

  1. A. Vershinin

    Present address: Karyobiology Group, John Innes Centre for Plant Science Research, Colney Lane, NR4 7UH, Norwich, UK

Authors and Affiliations

  1. Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-268 31, Svalöv, Sweden

    S. Svitashev, T. Bryngelsson & R. von Bothmer

  2. Siberian Department of Russian Academy of Sciences, Institute of Cytology and Genetics, Lavrentieva 10, 630090, Novosibirsk, Russia

    A. Vershinin

  3. Plant Biology Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000, Roskilde, Denmark

    C. Pedersen

  4. Department of Genetics, Lund University, S-22362, Lund, Sweden

    T. Säll

Authors
  1. S. Svitashev
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  2. T. Bryngelsson
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  3. A. Vershinin
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  4. C. Pedersen
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  5. T. Säll
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  6. R. von Bothmer
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Communicated by P. M. A. Tigerstedt

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Svitashev, S., Bryngelsson, T., Vershinin, A. et al. Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. Theoret. Appl. Genetics 89, 801–810 (1994). https://doi.org/10.1007/BF00224500

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