Abstract
A leucine/valine substitution at amino acid position 127 was identified by the polymerase chain reaction and restriction fragment length polymorphism in the bovine growth hormone gene. Genotyping was performed in 84 AI bulls of three different breeds, in which plasma concentrations of growth hormone (GH) and insulin-like growth factor I (IGF-1) were also measured. Gene frequencies of variants L (leucine) und V (valine) were 0.80/0.20 (Black and White), 0.90/0.10 (Brown), 0.71/0.29 (Simmental). Hormone concentrations were measured during different physiological conditions (normal feeding, fasting, realimentation) in the majority of animals. Generally, genotype LL was associated with higher concentrations of GH than LV. This difference was significant in Black and White bulls (P < 0.05). In contrast, IGF-1 concentrations were higher in LV than in LL animals. This was most pronounced in mature, realimented Simmental bulls. We conclude that the various GH alleles influence the circulating concentrations of GH and IGF-1.
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Arthur PF, Makarechian M, Salmon RK, Price MA (1990) Plasma growth hormone and insulin concentrations in double-muscled and normal bull calves. J Anim Sci 68:1609–1615
Chikuni K, Terada F, Kageyama S, Koishikawa T, Kato S, Ozutsumi K (1991) Identification of DNA sequence variants for amino acid residue 127 of bovine growth hormone using the polymerase chain reaction method. Anim Sci Technol (Jpn) 62:660–666
Davis ME, Bishop MD (1991) Preliminary results on between and within twin set variation in insulin-like growth factor I (IGF-1) and some relationships with performance traits in identical twin heifers. Livestock Prod Sci 27:255–262
Eppard PJ, Bentle LA, Violand BN, Ganguli S, Hintz RL, Kung L, Jr, Krivi GG, Lanza GM (1992) Comparison of the galactopoietic response to pituitary-derived and recombinant-derived variants of bovine growth hormone. J Endocrinol 132:47–56
Graml R, Weiss G, Buchberger J, Pirchner F (1989) Different rates of synthesis of whey protein and casein by alleles of the β-lactoglobulin and alpha SL -casein locus in cattle. Génét Sél Evol 21:547–554
Groenewegen PP, McBride BW, Burton JH, Elsasser TH (1990) Effect of bovine somatotropin on the growth rate, hormone profiles and carcass composition of Holstein bull calves. Dom Anim Endocrinol 7:43–54
Kazmer GW, Barnes MA, Akers RM, Pearson RE (1986) Effect of genetic selection for milk yield and increased milking frequency on plasma growth hormone and prolactin concentration in Hoistein cows. J Anim Sci 63:1220–1227
Kazmer GW, Canfield RW, Bean B (1991) Somatotropin and prolactin profile characteristics in proven AI dairy sires. J Anim Sci 69:1601–1606
Klemetsdal G, Tveit B, Vingelen M, Starova J, Sejrsen K (1991) The plasma level of growth hormone in two genetic lines of dairy cattle selected for high and low milk yield. 42 nd Annu Meeting EAAP, Berlin, September 1991, G5b7
Lucy MC, Hauser SD, Eppard PJ, Krivi GG, Collier RJ (1991) Genetic polymorphism within the bovine somatotropin (bST) gene detected by polymerase chain reaction and endonuclease digestion. J Dairy Sci 74 (Suppl 1):284
Ohlson DL, Koch RM, Klindt J, Davis SL (1987) Relationship of growth hormone, prolactin and thyrotropin secretion to individual and progeny performance of Hereford bulls. J Anim Sci 65:63–67
Robinson DL, Hammond K, Graser H-U, McDowell GH (1992) Relationships between breeding values and physiological responses to fasting and refeeding in dairy bulls. J Anim Breed Genet 109:26–36
Ronge H, Blum J, Clement C, Jans F, Leuenberger H, Binder H (1988) Somatomedin C in dairy cows related to energy and protein supply and to milk production. Anim Prod 47:165–183
Salmon RK, Berg RT, Yen FC, Hodgetts FB (1988) Identification of a variant growth hormone haplotype in mice selected for high body weight. Genet Res 52:7–15
Schams D, Graf F, Graule B, Abele M, Prokopp S (1991a) Hormonal changes during lactation in cows of three different breeds. Livestock Prod Sci 27:285–296
Schams D, Graf F, Meyer J, Graule B, Mauthner M, Wollny C (1991b) Changes in hormones, metabolites, and milk after treatment with sometribove (recombinant methionyl bST) in Deutsches Fleckvieh and German Black and White cows. J Anim Sci 69:1583–1592
Seavey BK, Singh RNP, Lewis UJ, Geschwind II (1971) Bovine growth hormone: evidence for two allelic forms. Biochem Biophys Res Commun 43:189–195
Walters DL, Schams D, Schallenberger E (1984) Pulsatile secretion of gonadotropins, ovarian steroids and ovarian oxytocin during the luteal phase of the oestrous cycle of the cow. J Reprod Fertil 71:479–491
Wheaton JE, Al-Raheem SN, Massri YG, Marcek JM (1986) Twentyfour-hour growth hormone profiles in Angus steers. J Anim Sci 62:1267–1272
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Schlee, P., Graml, R., Schallenberger, E. et al. Growth hormone and insulin-like growth factor I concentrations in bulls of various growth hormone genotypes. Theoret. Appl. Genetics 88, 497–500 (1994). https://doi.org/10.1007/BF00223667
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DOI: https://doi.org/10.1007/BF00223667