Abstract
Mitochondrial DNA (mtDNA) from 13 cytoplasmic male-sterile (cms) lines from diverse sources were characterized by Southern blot hybridization to pearl millet and maize mtDNA probes. Hybridization patterns of mtDNA digested with PstI, BamHI, SmaI or XhoI and probed with 13.6-, 10.9-, 9.7- or 4.7-kb pearl millet mtDNA clones revealed similarities among the cms lines 5141 A and ICMA 1 (classified as the S-A1 type of cytoplasm based on fertility restoration patterns), PMC 30A and ICMA 2. The remaining cms lines formed a distinct group, within which three subgroups were evident. Among the maize mitochondiral gene clones used, the coxI probe revealed two distinct groups of cytoplasms similar to the pearl millet mtDNA clones. The atp9 probe differentiated the cms line 81 A4, derived from P. glaucum subsp. monodii, while the coxII gene probe did not detect any polymorphism among the cms lines studied. MtDNA digested with BamHI, PstI or XhoI and hybridized to the atp6 probe revealed distinct differences among the cms lines. The maize atp6 gene clone identified four distinct cytoplasmic groups and four subgroups within a main group. The mtDNA fragments hybridized to the atp6 gene probe with differing intensities, suggesting the presence of more than one copy of the gene in different stoichiometries. Rearrangements involving the coxI and/or rrn18-rrn5 genes (mapped within the pearl millet clones) probably resulted in the S-A1 type of sterility. Rearrangements involving the atp6 gene (probably resulting in chimeric form) may be responsible for male sterility in other cms lines of pearl millet.
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References
Andrews DJ, Anand Kumar K (1982) Cytoplasmic male-sterility in pearl millet (Pennisetum americanum) (L.) (Leeke) — a review. Mutat Breed Newsl 20:1–3
Appa Rao S, Mengesha MH, Rajagopal Reddy C (1989) Development of cytoplasmic male-sterile lines of pearl millet from Ghana and Botswana germplasm. In: Manna GK, Sinha U (eds) Perspectives in cytology and genetics, vol 6, pp 817–823
Bailey-Serres J, Dixon LK, Liddell AD, Leaver CJ (1986) Nuclear-mitochondrial interaction in cytoplasmic male-sterile sorghum. Theor Appl Genet 73:252–260
Bookjans G, Stummann BM, Henningsen KW (1984) Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength. Anal Biochem 141:244–247
Borck KS, Walbot V (1982) Comparison of the restriction endonuclease digestion patterns of mitochondrial DNA from normal and male-sterile cytoplasms of Zea mays L. Genetics 102:109–128
Burton GW (1983) Breeding pearl millet. Plant Breed Rev 1:162–182
Buton GW, Athwal DS (1967) Two additional sources of cytoplasmic male sterility in pearl millet and their relationship to Tift 23A. Crop Sci 7:209–211
Dallaporta SL, Wood J, Hicks JB (1983) A plant minipreparation: version II. Plant Mol Biol Rep 4:19–21
Dewey RE, Levings CS, III, Timothy DH (1985a) Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79:914–919
Dewey RE, Schuster AM, Levings CS, III, Timothy DH (1985b) Nucleotide sequence of F0-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci USA 82:1015–1019
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Hanna WW (1987) A new stable cytoplasm in pearl millet. In: Agronomy abstracts. ASA, Madison Wis., p 64
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions conferring male-sterilities in plants. Int Rev Cytol 94:213–265
Isaac PG, Jones VP, Leaver CJ (1985) The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male-sterile plants. EMBO J 4:1617–1623
Marchais L, Pernes J (1985) Genetic divergence between wild and cultivated pearl millet (Pennisetum typhoides). I. Male sterility. Z Pflanz Zuecht 95:103–112
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Pokhriyal SC, Unnikrishnan KV, Balzar Singh, Ram Dass, Patil RR (1976) Induction of downy mildew resistance in pearl millet male-sterile Tift 23d2. Indian J Genet 36:403–409
Smith RL, Chowdhury MKU (1989) Mitochondrial DNA polymorphism in male-sterile and fertile cytoplasms of pearl millet. Crop Sci 29:809–814
Smith RL, Chowdhury MKU (1991) Characterisation of pearl millet mitochondrial DNA fragments rearranged by reversion from cytoplasmic male sterility to fertility. Theor Appl Genet 81:793–799
Smith RL, Chowdhury MKU, Pring DR (1987) Mitochondrial DNA rearrangements in Pennisetum associated with reversion from cytoplasmic male-sterility to fertility. Plant Mol Biol 9:277–286
Smith RL, Chowdhury MKU, Schank SL (1989) Use of restriction fragment length polymorphism (RFLP) markers in genetics and breeding of napiergrass. Soil Crop Sci Soc Fla Proc 48:13–19
Weihe A, Dudareva NA, Veprev SG, Maletsky SI, Melzer R, Salganik RI, Borner Th (1991) Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of sugar beet Beta vulgaris. Theor Appl Genet 82:11–16
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Rajeshwari, R., Sivaramakrishnan, S., Smith, R.L. et al. RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet. Theoret. Appl. Genetics 88, 441–448 (1994). https://doi.org/10.1007/BF00223658
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DOI: https://doi.org/10.1007/BF00223658