Summary
The ultrastructure of mitochondria in monkey myocardial cells was investigated by scanning electron microscopy, thin sections and freeze-fracturing. Mitochondria with well-developed cristae were distributed around the nucleus, between the myofibrils and beneath the sarcolemma. Those clustered near the the poles of the nucleus were generally spherical in shape. Interfibrillar mitochondia were arranged in longitudinal rows between the myofibrils, were elongated and usually about the same length as a sarcomere. Subsarcolemmal mitochondria varied in size and shape, being rod-like, spherical, polygonal or horseshoe-like. There were usually two profiles of subsarcolemmal mitochondria in each section of sarcomere, although sometimes one or three occurred, and they were typically oriented perpendicularly to the myofibrils. These morphological differences among mitochondria could reflect functional and/or mechanical properties in the various cellular locations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyde A (1978) Pros and cons of critical point drying and freeze drying for SEM. IITRI/SEM/1978 pp 303–314
Evan AP, Dail WG, Dammrose D, Palmer C (1976) Scanning electron microscopy of cell surfaces following removal of extracellular material. Anat Rec 185:433–446
Haggis GH, Bond EF, Phipps B (1974) Visualization of mitochondrial cristae and nuclear chromatin by SEM. IITRI/SEM/1976 pp 275–281
Hoppel CL, Tandler W, Parland W, Turkaly JS, Albers LO (1982) Hamster cardiomyopathy: A defect in oxidative phosphorylation in the cardiac interfibrillar mitochondria. J Biol Chem 257:1540–1548
Kubišta V, Kubištova J, Pette D (1971) Thyroid hormone induced changes in enzyme activity pattern of energy-supplying metabolism of fast (white), slow (red), and heart muscle of the rat. Eur J Biochem 18:553–560
McCallister EP, Page E (1973) Effects of thyroxin on ultrastructure of rat myocardial cells: a stereological study. J Ultrastruct Res 42:136–155
Müller W (1976) Subsarcolemmal mitochondria and capillarization of soleus muscle fibers in young rats subjected to an endurance training. Cell Tissue Res 174:367–389
Myklebust RH, Dalen H, Saetersdal TS (1980) A correlative transmission and scanning electron microscopic study of the pigeon myocardial cell. Cell Tissue Res 207:31–41
Palmer JW, Tandler B, Hoppel CL (1977) Biochemical properties of subsarcolemmal and interfibrillar mitochondria isolated from rat cardiac muscle. J Biol Chem 252:8731–8739
Romanul FCA (1965) Capillary supply and metabolism of muscle fibers. Arch Neurol 12:497–509
Segretain D, Ramourg A, Clermont Y (1981) Theree dimensional arrangement of mitochondria and endoplasmic reticulum in the heart muscle fiber of the rat. Anat Rec 200:139–151
Sjöstrand FS, Cassel RZ (1978) Strcuture of inner membrane in rat heart muscle mitochondria as revealed by means of freezefracturing. J Ultrastruct Res 63:111–137
Sjöstrand FS, Cassel RZ (1978) The structure of the surface membranes in rat heart muscle mitochondria as revealed by freezefracturing. J Ultrastruct Res 63:138–154
Stenger RJ, Spiro D (1961) The ultrastructure of mammalian cardiac muscle. J Biophys Biochem Cytol 9:325–351
Thiery G, Rambourg A (1976) A new staining technique for studying thick sections in the electron microscope. J Microsc Biol Cell 26:103–106
Tomanek RJ, Karlsson UL (1973) Myocardial ultrastructure of young and senescent rats. J Ultrastruct Res 42:201–220
Vahouney GV, Tamboli A, Maten MV, Albert N (1979) Morphological and metabolic studies on adult cardiac myocytes. IITRI/ SEM/1979 pp 375–388
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimada, T., Horita, K., Murakami, M. et al. Morphological studies of different mitochondrial populations in monkey myocardial cells. Cell Tissue Res. 238, 577–582 (1984). https://doi.org/10.1007/BF00219874
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00219874