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Assignment of the human CRABP-II gene to chromosome 1q21 by nonisotopic in situ hybridization

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Summary

Two highly conserved forms of cellular retinoic acid binding protein (CRABP-I and CRABP-II) have been described, and one, CRABP-II, is highly expressed in human skin. We have utilized a 10-kb fragment containing the human CRABP-II (hCRABP-II) gene (isolated from a human genomic library) to localize hCRABP-II to human chromosome 1 band q21 by fluorescence in situ hybridization. Localization to 1q was confirmed by hybridization of a hCRABP-II cDNA clone against a human-mouse hybrid cell line containing a t(1;6)(q21;q13) translocation chromosome. The hCRABP-II gene is therefore localized to a band known to contain several other genes that are expressed in the context of epidermal differentiation, including profilaggrin, loricrin, involucrin, and calcyclin.

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Authors and Affiliations

  1. Department of Dermatology, The University of Michigan Medical School, 1150 West Medical Center Drive, 48109-0668, Ann Arbor, MI, USA

    J. T. Elder, A. Åström, U. Pettersson & J. J. Voorhees

  2. Department of Radiation Oncology and Human Genetics, The University of Michigan Medical School, 1150 West Medical Center Drive, 48109-0668, Ann Arbor, MI, USA

    J. M. Trent

Authors
  1. J. T. Elder
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  2. A. Åström
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  3. U. Pettersson
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  4. J. J. Voorhees
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  5. J. M. Trent
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Elder, J.T., Åström, A., Pettersson, U. et al. Assignment of the human CRABP-II gene to chromosome 1q21 by nonisotopic in situ hybridization. Hum Genet 89, 487–490 (1992). https://doi.org/10.1007/BF00219171

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  • DOI: https://doi.org/10.1007/BF00219171

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