Skip to main content
Log in

Synthesis and distribution of laminin-related polypeptides in early amphibian embryos

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Western blotting experiments carried out with several heterospecific antibodies against mouse-derived laminin allowed the identification of four laminin-related polypeptides in early Pleurodeles waltlii embryos. Synthesis of all four polypeptides was detected from the early blastula stage to late gastrula stage. Immunofluorescent staining with anti-laminin and anti-fibronectin antibodies provided evidence for a close association of these laminin-related polypeptides with the fibronectin fibrillar network.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ballantine JEM, Woodland HR, Strurgess EA (1979) Changes in protein synthesis during the development of Xenopus laevis. J Embryol Exp Morphol 51:137–153

    Google Scholar 

  • Boucaut JC, Darribère T (1983) Fibronectin in early amphibian embryos. Migrating mesodermal cells contact fibronectin established prior to gastrulation. Cell Tissue Res 234:135–145

    Google Scholar 

  • Boucaut JC, Darribère T, Boulekbache H, Thiery JP (1984a) Prevention of gastrulation but not neurulation by antibodies to fibronectin in amphibian embryos. Nature 307:364–367

    Google Scholar 

  • Boucaut JC, Darribère T, Poole TJ, Aoyama H, Yamada KM, Thiery JP (1984b) Biologically active synthetic peptides as probe of embryonic development: a competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian and neural crest cell migration in avian embryos. J Cell Biol 99:1822–1830

    Google Scholar 

  • Boucaut JC, Darribère T, Shi De Li, Boulekbache H, Yamada KM, and Thiery JP (1985) Evidence for the role of fibronectin in amphibian gastrulation. J Embryol Exp Morphol [Suppl] 89:211–227

    Google Scholar 

  • Chung AE, Jaffe R, Bender B, Lewis M, Durkin M (1983) Monoclonal antibodies against the GP-2 subunit of laminin. Lab Invest 49:576–581

    Google Scholar 

  • Cooper AR, Kurkinen M, Taylor A, Hogan BLM (1981) Studies on the biosynthesis of laminin by murine parietal endoderm cells. Eur J Biochem 119:189–197

    Google Scholar 

  • Cooper AR, McQueen HA (1983) Subunits of laminin are differentially synthesized in mouse eggs and early embryos. Dev Biol 96:467–471

    Google Scholar 

  • Darribère T, Boucher D, Lacroix JC, Boucaut JC (1984) Fibronectin synthesis in the early embryogenesis of Pleurodeles waltlii Cell Differ 14:171–177

    Google Scholar 

  • Darribère T, Boulekbache H, Shi De Li, Boucaut JC (1985) Immuno-electron-microscopic study of fibronectin in gastrulating amphibian embryos. Cell Tissue Res 239:75–80

    Google Scholar 

  • Dreyer C, Hausen P (1983) Two dimensional gel analysis of the fate of oocyte nuclear proteins in the development of Xenopus laevis. Dev Biol 100:412–425

    Google Scholar 

  • Engel J, Odermatt E, Engel A, Madri JA, Furthmayr H, Rohde H, Timpl R (1981) Shapes, domain organization and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150:97–120

    Google Scholar 

  • Foidart JM, Bere EW, Yaar M, Rennard SI, Gullion M, Martin GR, Katz SI (1980) Distribution and immunoelectron microscopic localization of laminin, a non collagenous basement membrane glycoprotein. Lab Invest 42:336–342

    Google Scholar 

  • Gallien L, Durocher M (1957) Table chronologique du développement chez Pleurodeles waltlii Michah. Bull Biol, France et Belgique 91:97–114

    Google Scholar 

  • Heifetz A, Snyder JM (1981) The effect of hydrocortisone on the biosynthesis of sulfated glycoconjugates by human fetal lung. J Biol Chem 256:4957–4967

    Google Scholar 

  • Holtfreter J (1943) A study of the mechanisms of gastrulation II. J Exp Zool 95:171–212

    Google Scholar 

  • Howe CC, Dietzschold B (1983) Structural analysis of three subunits of laminin from teratocarcinoma-derived parietal endoderm cells. Dev Biol 98:385–391

    Google Scholar 

  • Johnson KE (1977) Extracellular matrix synthesis in blastula and gastrula stages of normal and hybrid frog embryos. III Characterization of galactose- and glucosamine labelled material. J Cell Sci 25:335–354

    Google Scholar 

  • Keller RE (1975) Vital dye mapping of the gastrula and neurula of Xenopus laevis. I Prospective areas and morphogenetic movements of the superficial layer. Dev Biol 42:222–241

    Google Scholar 

  • Keller RE (1976) Vital dye mapping of the gastrula and neurula of Xenopus laevis. II Prospective areas and morphogenetic movements of the deep layer. Dev Biol 51:118–137

    Google Scholar 

  • Keller RE (1978) Time-lapse cinemicrographic analysis of superficial cell behavior during and prior to gastrulation in Xenopus laevis. J Morphol 157:223–248

    Google Scholar 

  • Keller RE, Danilchik M, Gimlich RL, Shih J (1985) The function and mechanism of convergent extension during gastrulation of Xenopus laevis. J Embryol Exp Morphol [Suppl] 89:185–209

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–689

    Google Scholar 

  • Lee G, Hynes R, Kirschner M (1984) Temporal and spatial regulation of fibronectin in early Xenopus development. Cell 36:729–740

    Google Scholar 

  • Leivo I, Vaheri A, Timpl R, Wartiovaara J (1980) Appearance and distribution of collagens and laminin in the early mouse embryo. Dev Biol 76:100–114

    Google Scholar 

  • Muillerman HG, Ter Hart HGJ, Van Dyk W (1982) Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme immuno assay method using unspecific antiserum and partially purified active enzyme: application to rat liver phosphodiesterase I. Anal Biochem 120:46–51

    Google Scholar 

  • Nakatsuji N, Johnson KE (1983) Comparative study of extracellular fibrils on the ectodermal layer in gastrulae of five amphibian species. J Cell Sci 59:61–70

    Google Scholar 

  • Nakatsuji N, Smolira MA, Wylie CC (1985a) Fibronectin visualized by scanning electron microscopy immunocytochemistry on the substratum for cell migration in Xenopus laevis gastrulae. Dev Biol 107:264–268

    Google Scholar 

  • Nakatsuji N, Hashimoto K, Hayashi M (1985b) Laminin fibrils in newt gastrulae visualized by the immunofluorescent staining. Dev Growth Differ 27:639–643

    Google Scholar 

  • Sakashita S, Engvall E, Ruoslahti E (1980) Basement membrane laminin binds to heparin. FEBS Lett 116:243–246

    Google Scholar 

  • Steinberg MS (1957) In: Carnegie Inst Washington Year Book 56. Cited by JB Ebert

  • Terranova VP, Rohrbach DH, Martin GR (1980) Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen. Cell 22:719–726

    Google Scholar 

  • Timpl R, Rohde H, Gehron Robey P, Rennard SI, Foidart JP, Martin GR (1979) Laminin. A glycoprotein from basement membranes. J Biol Chem 254:8922–8927

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Vogt W (1929) Gestaltungsanalyse am Amphibienkeim mit örtlicher Vitalfärbung. II Gastrulation und Mesodermbildung bei Urodelen und Anuren. Arch Entwickl Mech Org 120:384–706

    Google Scholar 

  • Yamada KM (1983) Cell surface interactions with extracellular materials. Ann Rev Biochem 52:761–799

    Google Scholar 

  • Yurchenco PD, Tsilibary EC, Charonis AS, Furthmayr H (1985) Laminin polymerization in vitro. Evidence for a two-step assembly with domain specificity. J Biol Chem 260:7636–7644

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Darribère, T., Riou, JF., De Shi, L. et al. Synthesis and distribution of laminin-related polypeptides in early amphibian embryos. Cell Tissue Res. 246, 45–51 (1986). https://doi.org/10.1007/BF00218997

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00218997

Key words

Navigation