Abstract
A study of poliomyelitis neutralizing antibodies was carried out on 165 samples of blood serum drawn from subjects aged 14–15 years who had completed vaccination with OPV 11 or 12 years ago.
Two different methods of incubation of the serum-virus mixtures (1 hour at 37°C; 6 hours at 37°C and then 18 hours at 4°C, respectively) and two different cell lines (RC-37 and Hep-2) for the inoculation of these mixtures were employed.
The results were also evaluated in relation to different initial dilutions of the sera (from 1:4 to 1:1).
With the 1:4 initial serum dilution the highest frequencies of sero-negativity were observed utilizing the short incubation time and inoculation in Hep-2 (42% for polio 1,10% for polio 2, 32% for polio 3). These frequencies diminish significantly utilizing the RC-37 cell line and the long incubation respectively.
Under all the experimental conditions the results were better when the 1:2 initial serum dilutions were employed and even better when undiluted sera were used. Neutralizing antibodies against the 3 types of poliovirus were detected in the undiluted sera of all the subjects utilizing the long incubation and inoculation in either the RC-37 or the Hep-2 cell lines.
In three subjects lacking detectable antibodies at the 1:4 dilution of the sera, the administration of a dose of IPV produced after 5–7 days a high response which remained almost unchanged 30 days later.
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Bellelli, E., Tanzi, M.L., Bocelli, V. et al. Antipoliomyelitis immunity status among a population that was regularly vaccinated 11–12 years before. Eur J Epidemiol 7, 605–611 (1991). https://doi.org/10.1007/BF00218669
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DOI: https://doi.org/10.1007/BF00218669