Summary
Polymorphism of an endogenous α-amylase inhibitor in wheat was studied using iso-electric focusing followed by monoclonal antibody — based immunoblotting. Ten isoforms of the inhibitor detected in common wheat and its wild counterparts were assigned to five homoeologous loci. Three α-amylase inhibitor loci (Isa-1) were identified in common wheat and located on the long arms of chromosomes 2A, 2B and 2D. In a sample of 27 bread wheats, eight durum wheats, and 12 diploid wheat relatives, amphiploids and triticales, a high resolution isoelectric-focusing separation demonstrated two active and one null allele at the Isa-A1, two alleles at the Isa-B1, one allele at the Isa-D1, four alleles at the Isa-S1, and one allele at the Isa-G1 locus. The most frequent electrophoretic pattern of common wheat cultivars consisted of two isoforms, encoded respectively by the Isa-B1b, Isa-D1 a alleles and the Isa-Alnull allele. All the durum wheats had only one inhibitor form controlled by allele Isa-B1b, which was accompanied by the null allele at the Isa-A1 locus.
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Communicated by J. W. Snape
Contribution No. 210 of the Food Science Department, University of Manitoba
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Masojć, P., Zawistowski, J., Howes, N.K. et al. Polymorphism and chromosomal location of endogenous α-amylase inhibitor genes in common wheat. Theoret. Appl. Genetics 85, 1043–1048 (1993). https://doi.org/10.1007/BF00215045
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DOI: https://doi.org/10.1007/BF00215045