Abstract
The ATF-1 gene encodes for a transcription factor normally regulated by cAMP (Hai et al. 1989, Yoshimura et al. 1990). Recently, it has been shown to be involved in the recurrent t(12;22) translocation observed in soft tissue malignant melanoma, in a fusion gene with the EWS gene (Zucman et al. 1993). We report here on its precise localization on chromosome 12 by fluorescence in situ hybridization.
References
Aman P, Ron D, Mandahl N, Fioretos T, Heim S, Arheden K, Willén H, Rydholm A, Mitelman F (1992) Rearrangement of the transcription factor gene CHOP in myxoid liposarcomas with t(12;16)(qa13;p11). Genes Chromosom Cancer 5:278–285
Desmaze C, Zucman J, Delattre O, Thomas G, Aurias A (1992) Unicolor and bicolor in situ hybridization in the diagnosis of peripheral neuroepithelioma and related tumors. Genes Chromosom Cancer 5:30–34
Desmaze C, Scambler P, Prieur M, Halford S, Sidi D, Le Deist F, Aurias A (1993) Routine diagnosis of DiGeorge syndrome by fluorescent in situ hybridization. Hum Genet 90:663–665
Hai T, Liu F, Coukos WJ, Green MR (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. Genes Dev 3:2083–2090
Johnson GD, Nogueira Araujo GM de C (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Method 43:349–350
Lemieux N, Dutrillaux B, Viegas-Péquignot E (1992) A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes. Cytogenet Cell Genet 59:311–312
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Yoshimura T, Fijisawa J-I, Yoshida M (1990) Multiple cDNA clones encoding nuclear proteins that bind to the tax-dependent enhancer of HTLV-1: all contain a leucine zipper structure and basic amino acid domain. EMBO J 9:2537–2542
Zucman J, Delattre O, Desmaze C, Epstein A, Stenman G, Speleman F, Fletcher CDM, Aurias A, Thomas G (1993) EWS and ATF-1 gene fusion induced by the recurrent t(12;22) translocation in malignant melanoma of soft parts. Nature [Genet] 4:341–345
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Desmaze, C., Zucman, J., Delattre, O. et al. Precise localization on chromosome 12 of the ATF-1 gene by fluorescence in situ hybridization. Hum Genet 93, 207–208 (1994). https://doi.org/10.1007/BF00210613
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00210613