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Parvoviruses are inefficient in inducing interferon-β, tumor necrosis factor-α, or interleukin-6 in mammalian cells

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Abstract

To investigate a possible role of cytokines in parvovirus-mediated suppression of tumorigenesis, we tested in cell culture whether parvoviruses are able to induce interferon (IFN)-β, tumor necrosis factor (TNF)-α or interleukin-6 (IL-6). Infection of rodent or human cells with the parvoviruses minute virus of mice (MVM), H-1 or adeno-associated virus (AAV) types 2 or 5 failed to induce expression of the luciferase or β-galactosidase reporter genes transfected into these cells as constructs containing an IFN-β promoter. Parvoviruses did weakly induce synthesis of TNF-α and of IL-6 in cell culture and could slightly enhance synthesis of these cytokines when induced by other agents. These in vitro data suggest that the rather unspecific tumor-suppressive properties of parvoviruses are unlikely to be attributable to stimulation of the synthesis of IFN, TNF or IL-6.

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Author notes

  1. Martin Rentrop

    Present address: Institut für Physiologische Chemie, Johannes-Gutenberg-Universität Mainz, Duesbergweg 6, W-6500, Mainz, FRG

  2. Daniela N. Männel

    Present address: Institut für Pathologie und Immunologie, Klinikum der Universität Regensburg, Franz-Josef-Strauss-Allee, W-8400, Regensburg, FRG

Authors and Affiliations

  1. Angewandte Tumorvirologie, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany

    Jörg R. Schlehofer & Martin Rentrop

  2. Tumorimmunologie, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany

    Daniela N. Männel

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Schlehofer, J.R., Rentrop, M. & Männel, D.N. Parvoviruses are inefficient in inducing interferon-β, tumor necrosis factor-α, or interleukin-6 in mammalian cells. Med Microbiol Immunol 181, 153–164 (1992). https://doi.org/10.1007/BF00202055

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