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Effect of Nocardia rubra cell wall skeleton on augmentation of cytotoxicity function in human pleural macrophages

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Summary

The ability of Nocardia rubra cell wall skeleton (N-CWS) to augment macrophage cytotoxicity function was examined using human pleural macrophages prepared from 32 malignant pleural effusions and 53 pleural washings. The cytostatic activity of pleural macrophages for human lung cancer cells (PC-9) was augmented following incubation of pleural mononuclear cells with 10 μg/ml N-CWS for 24 h. Macrophage activity was increased by direct interaction of macrophages with N-CWS or by incubation of macrophages with supernatant culture fluids from pleural lymphocytes with N-CWS. The cytotoxic potential of the pleural macrophages obtained from patients treated with 500 μg of N-CWS intrapleurally was also increased. The heat and acid stability studies revealed that the culture fluids from pleural lymphocytes treated with N-CWS contained macrophage activation factor in addition to interferon-γ. These results suggest that direct and indirect macrophage activation is part of the mechanism in which N-CWS has a clinical effect on malignant pleural effusions.

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Authors and Affiliations

  1. Department of Internal Medicine, National Kinki-Chuo Hospital for Chest diseases, 1180 Nagasone-Cho, Sakai, 591, Osaka, Japan

    Mitsunori Sakatani

  2. Tokushima University Medical School, Kuramoto-Cho, 770, Tokushima, Japan

    Takeshi Ogura

  3. Osaka University Medical School, Fukushima-Ku, 553, Osaka, Japan

    Tomiya Masuno & Susumu Kishimoto

  4. Osaka University, Kamiyamada Suita, 565, Osaka, Japan

    Yuichi Yamamura

Authors
  1. Mitsunori Sakatani
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  2. Takeshi Ogura
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  3. Tomiya Masuno
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  4. Susumu Kishimoto
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  5. Yuichi Yamamura
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Sakatani, M., Ogura, T., Masuno, T. et al. Effect of Nocardia rubra cell wall skeleton on augmentation of cytotoxicity function in human pleural macrophages. Cancer Immunol Immunother 25, 119–125 (1987). https://doi.org/10.1007/BF00199951

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  • DOI: https://doi.org/10.1007/BF00199951

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