Skip to main content
SpringerLink
Log in

Further studies on the differences in cytotoxicity of human peripheral blood monocytes and bronchoalveolar macrophages for cultured human lung cells

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Previously reported differences between the cytostatic activity of human peripheral blood monocytes (PBM) and bronchoalveolar macrophages (BAM) for cultured human lung tumor cells have been further investigated. The differences are both quantitative and qualitative and are shown not to be due to the respective methods of purification. There was a varying contribution of cytolysis to the cytostasis detected by the 75selenomethionine post-labeling assay used. Bronchoalveolar macrophages were cytolytic when tested at both low and high E: T ratios but PBM were only cytolytic at the low E: T ratio. A variable dependence upon soluble cytostatic factor(s) was suggested, and there was evidence of heterogeneity in the factors released by the two populations. Cytostatic factor production by both populations appeared to be under similar regulatory constraints. In vitro maturation of PBM altered their cytostatic dose-response curve to one resembling that previously reported for BAM. It was also shown that sera from poor-prognosis lung tumor patients, which suppressed the in vitro maturation of PBM, also suppressed the in vitro cytostatic activity of PBM for cultured human lung tumor cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Balkwell FR, Hogg N (1979) Characterisation of human breast milk macrophages cytostatic for human cell lines. J Immunol 123:1451

    Google Scholar 

  2. Binderup L, Bramm E, Arrigone-Martelli E (1978) Peritoneal macrophages from adjuvant arthritis rats enhance tumor cell growth in vitro. Experientia 35:1230

    Google Scholar 

  3. Blusse van oud Alblas A, van Furth R (1979) Origin, kinetics and characteristics of pulmonary macrophages in the normal steady state. J Exp Med 149:1504

    Google Scholar 

  4. Cabilly S, Gallily R (1977) Studies on the recognition of xenogeneic cells by non-immune macrophages. I. Destruction of chicken fibroblasts in vitro by murine macrophages. Cell Immunol 29:54

    Google Scholar 

  5. Cameron DJ, Hallowell Churchill W (1979) Cytotoxicity of human macrophages for tumor cells: enhancement by human lymphocyte mediators. J Clin Invest 63:977

    Google Scholar 

  6. Cameron DJ, Hallowell Churchill W (1980) Cytotoxicity of human macrophages for tumor cells: enhancement by bacterial lipopolysaccharides (LPS). J Immunol 124:708

    Google Scholar 

  7. Coggle JE, Tarling JD (1982) Cell kinetics of pulmonary alveolar macrophages in the mouse. Cell Tissue Kinet 15:139

    Google Scholar 

  8. Cohen AB, Cline MJ (1972) In vitro studies of the foamy macrophage of postobstructive endogenous lipoid pneumonia in man. Am Rev Respir Dis 106:69

    Google Scholar 

  9. DeLustro F, Sherer GK, LeRoy EC (1980) Human monocyte stimulation of fibroblast growth by a soluble mediator(s). J Reticuloendothel Soc 28:519

    Google Scholar 

  10. Dent R, Cole P (1981) In vitro monocyte maturation as a prediction of survival in squamous cell carcinoma of the lung. Thorax 36:446

    Google Scholar 

  11. Dent RG, Cole PJ (1982) In vitro monocyte maturation in squamous-cell carcinoma of the lung: influence of humoral factors. Br J Cancer 46:522

    Google Scholar 

  12. Fray A, Lorinet AM, Thobie N, Halpern B (1979) Equal sensitivity of normal or tumoural fibroblasts to cytotoxic and cytostatic effects of normal or Corynebacterium parvum-activated peritoneal exudate cells. Ann Immunol [C] 130:445

    Google Scholar 

  13. Goldman R, Bar-Shavit Z (1979) Dual effect of normal and stimulated macrophages and their conditioned media on target cell proliferation. J Natl Cancer Inst 63:1009

    Google Scholar 

  14. Goldman R, Hogg N (1978) Enhanced susceptibility of virus-infected fibroblasts to cytostasis mediated by peritoneal exudate cells. J Immunol 121:1657

    Google Scholar 

  15. Hammerstrom J (1979) Human macrophage differentiation in vivo and in vitro. A comparison of human peritoneal macrophages and monocytes. Acta Pathol Microbiol Scand [C] 87:113

    Google Scholar 

  16. Hammerstrom J (1981) Human adherent mononuclear blood cells; cytolytic and cytostatic activity and characterisation of effector cells during in vitro culture. Acta Pathol Microbiol Scand [C] 88:201

    Google Scholar 

  17. Johnson KJ, Ward PA, Striker G, Kunkel R (1980) A study of the origin of pulmonary macrophages using the Chediak-Higashi marker. Am J Pathol 101:365

    Google Scholar 

  18. Lawson ADG, Stevenson GT (1983) Macrophages induce antibody-dependent cytostasis but not lysis in guinea pig leukaemic cells. Br J Cancer 48:227

    Google Scholar 

  19. Lemarbre P, Hoidal J, Vessella R, Rinehart J (1980) Human pulmonary macrophage tumor cell cytotoxicity. Blood 55:612

    Google Scholar 

  20. Liebovich SJ, Ross R (1976) A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro. Am J Pathol 84:501

    Google Scholar 

  21. Mannel DN, Falk W, Meltzer MS (1981) Inhibition of nonspecific tumoricidal activity by activated macrophages with antiserum against a soluble cytotoxic factor. Infect Immun 33:156

    Google Scholar 

  22. Mantovani A (1981) In vitro effects on tumor cells of macrophages isolated from an early-passage chemically induced murine sarcoma and from its spontaneous metastases. Int J Cancer 27:221

    Google Scholar 

  23. Mantovani A, Jerrells TR, Dean JH, Herberman RH (1979a) Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer 23:18

    Google Scholar 

  24. Mantovani A, Tagliabue A, Dean JH, Jerrells TR, Herberman RB (1979b) Cytolytic activity of circulating human monocytes on transformed and untransformed human fibroblasts. Int J Cancer 23:28

    Google Scholar 

  25. Matthews N (1981) Production of an anti-tumour cytotoxin by human monocytes. Immunology 44:135

    Google Scholar 

  26. Matthews N (1983) Effect on human monocyte killing of tumour cells of antibody raised against an extracellular monocyte cytotoxin. Immunology 48:321

    Google Scholar 

  27. Pertoft H, Johnsson A, Warmegard B, Seljelid R (1980) Separation of human monocytes on density gradients of Percoll. J Immunol Methods 33:221

    Google Scholar 

  28. Reidarson TH, Levy WE, Klostergaard J, Granger GA (1982) Inducible macrophage cytotoxins. I. Biokinetics of activation and release in vitro. JNCI 69:867

    Google Scholar 

  29. Rinehart J, Lange P, Gormus J, Kaplan ME (1978) Human monocyte-induced tumor cell cytotoxicity. Blood 52:211

    Google Scholar 

  30. Sone S, Tsubura E (1982) Human alveolar macrophages: potentiation of their tumoricidal activity by liposome-encapsulated muramyl dipeptide. J Immunol 129:1313

    Google Scholar 

  31. Sone S, Moriguchi S, Shimizu E, Ogushi F, Tsubura E (1982) In vitro generation of tumoricidal properties in human alveolar macrophages following interaction with endotoxin. Cancer Res 42:2227

    Google Scholar 

  32. Stewart CC, Beetham KC (1978) Cytocidal activity and proliferative ability of macrophages infiltrating the EMT6 tumor. Int J Cancer 22:152

    Google Scholar 

  33. Swinburne S, Cole PJ (1982) Human peripheral blood monocyte and bronchoalveolar macrophage cytotoxicity for cultured human lung tumor cells. J Reticuloendothel Soc 32:371

    Google Scholar 

  34. Swinburne S, Moore M, Cole P (1982) Human bronchoalveolar macrophage cytotoxicity for cultured human lung tumour cells. Br J Cancer 46:625

    Google Scholar 

  35. van Furth R, Blusse van oud Alblas A (1982) The current view on the origin of pulmonary macrophages. Pathol Res Pract 175:38

    Google Scholar 

Download references

Author information

Author notes

  1. Stuart Swinburne

    Present address: Department of Immunology, St. Mary's Hospital Medical School, Paddington, W2 1PG, London, England

Authors and Affiliations

  1. Host Defence Unit, Department of Medicine, Cardiothoracic Institute, Brompton Hospital, London, England

    Stuart Swinburne

  2. Department of Immunology, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, England

    Michael Moore & Peter Cole

Authors
  1. Stuart Swinburne
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Cole
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Swinburne, S., Moore, M. & Cole, P. Further studies on the differences in cytotoxicity of human peripheral blood monocytes and bronchoalveolar macrophages for cultured human lung cells. Cancer Immunol Immunother 19, 62–67 (1985). https://doi.org/10.1007/BF00199314

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00199314

Keywords

Search

Navigation