Skip to main content
SpringerLink
Log in

Synthesis of betanin from betanidin and UDP-glucose by a protein preparation from cell suspension cultures of Dorotheanthus bellidiformis (Burm. f.) N. E. Br.

  • Short Communication
  • Published:
Planta Aims and scope Submit manuscript

Abstract

Protein preparations from cell-suspension cultures of Dorotheanthus bellidiformis (Aizoaceae) catalyzed the formation of betanin (betanidin 5-O-glucoside) from betanidin and uridine 5′-diphosphate(UDP)-glucose. The enzyme activity can be classified as UDP-glucose: betanidin 5-O-β-glucosyltransferase (EC 2.4.1).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

HPLC:

high-performance liquid chromatography

References

  • Bokern, M. (1991) Strukturen und Stoffwechsel phenolischer Konjugate in betacyanführenden Zellkulturen von Chenopodium rubrum und Beta vulgaris. Dissertation, Technische Universität Braunschweig, FRG

    Google Scholar 

  • Bokern, M., Strack, D. (1988) Synthesis of hydroxycinnamic acid esters of betacyanins via 1-O-acylglucosides of hydroxycinnamic acids by protein preparations from cell suspension cultures of Chenopodium rubrum and petals of Lampranthus sociorum. Planta 174, 101–105

    Google Scholar 

  • Bokern, M., Wray, V., Strack, D. (1991) Accumulation of phenolic acid conjugates and betacyanins, and changes in the activities of enzymes involved in feruloylglucose metabolism in cell-suspension cultures of Chenopodium rubrum L. Planta 184, 261–270

    Google Scholar 

  • Bokern, M., Heuer, S., Strack, D. (1992) Hydroxycinnamic acid transferases in the biosynthesis of acylated betacyanins: purification and characterization from cell cultures of Chenopodium rubrum and occurrence in some other members of the Caryophyllales. Bot. Acta, in press

  • Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 3229–3231

    Google Scholar 

  • Elliott, D.C., Schultz, C.G., Cassar, R.A. (1983) Betacyanin decolourizing enzyme in Amaranthus tricolor seedlings. Phytochemistry 22, 383–387

    Google Scholar 

  • Girod, P.-A., Zryd, J.-P. (1991) Biogenesis of betalains: purification and partial characterization of DOPA-4,5-dioxygenase from Amanita muscaria. Phytochemistry 30, 169–174

    Google Scholar 

  • Heller, W., Forkmann, G. (1988) Biosynthesis. In: The flavonoids: Advances in research since 1980, pp. 399–425, Harborne, J.B., ed. Chapman and Hall, London

    Google Scholar 

  • Heuer, S., Wray, V., Metzger, J.W., Strack, D. (1992) Betacyanins from flowers of Gomphrena globosa. Phytochemistry, in press

  • Linsmaier, E.M., Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127

    Google Scholar 

  • Minale, L., Piattelli, S., De Stefano, S. (1967) Pigments of Centrospermae — VII. Betacyanins from Gomphrena globosa L. Phytochemistry 6, 703–709

    Google Scholar 

  • Steglich, W., Strack, D. (1990) Betalains. In: The alkaloids, vol. 39, pp. 1–62, Brossi, A., ed. Academic Press, London

    Google Scholar 

  • Strack, D., Marxen, N., Reznik, H., Ihlenfeld, H.-D. (1990) Distribution of betacyanins and hydroxycinnamic acid-glucose esters in flowers of the Ruschieae. Phytochemistry 29, 2175–2178

    Google Scholar 

  • Terradas, F., Wyler, H. (1991a) 2,3- and 4,5-Secodopas, the biosynthetic intermediates generated from l-dopa by an enzyme system extracted from the fly agaric, Amanita muscaria L., and their spontaneous conversion to muscaflavin and betalamic acid, respectively, and betalains. Helv. Chim. Acta 74, 124–140

    Google Scholar 

  • Terradas, F., Wyler, H. (1991b) The secodopas, natural pigments in Hygrocybe conica and in Amanita muscaria. Phytochemistry 30, 3251–3253

    Google Scholar 

  • Wyler, H., Dreiding, A.S. (1961) Über Betacyane, die stickstoffhaltigen Farbstoffe der Centrospermen. Vorl. Mitt. Experientia 17, 23–25

    Google Scholar 

  • Wyler, H., Meuer, U. (1979) Zur Biogenese der Betacyane: Versuche mit [2-14C]-Dopaxanthin. Helv. Chim. Acta 62, 1330–1339

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, Mendelssohnstrasse 1, W-3300, Braunschweig, Germany

    Susanne Heuer & Dieter Strack

Authors
  1. Susanne Heuer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dieter Strack
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the Deutsche Forschungsgemeinschaft (Bonn) and the Fonds der Chemischen Industrie (Frankfurt, FRG). Our special thanks are due to H. Böhm (Zentralinstitut für Ernährung, Potsdam-Rehbrücke, FRG) for generously providing Dorotheanthus callus cultures, and Christiane Forth for dependable technical assistance in establishing the cell suspension cultures. We also thank Maria Bokern for a gift of betanidin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heuer, S., Strack, D. Synthesis of betanin from betanidin and UDP-glucose by a protein preparation from cell suspension cultures of Dorotheanthus bellidiformis (Burm. f.) N. E. Br.. Planta 186, 626–628 (1992). https://doi.org/10.1007/BF00198045

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00198045

Key words

Search

Navigation