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Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina

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Abstract

A unique variant strain of Chara corallina, which contains little inorganic phosphate in the vacuole ([Pi]v) was isolated. The level of cytoplasmic inorganic phosphate ([Pi]c) in these cells was the same as that in normal cells. Using these unique cells, we studied the change in [Pi]c and the effect of Pi on the activities of electrogenic H+-pumps associated with the plasma membrane and tonoplast. Upon illumination, the plasma membrane of C. corallina became hyperpolarized by 15 mV, the pH of the vacuolar sap decreased by 0.5 unit, and [Pi]c decreased by 30% with a similar time course. The activities of the electrogenic H +-pump in the plasma membrane and the ATP and PPi-dependent H+-transport in the tonoplast were noncompetitively inhibited by Pi with Ki values of, in the order given, 21.3 mM, 22.1 mM and 37.7 mM. From the kinetics study we calculated that the electrogenic H+-pump in the plasma membrane and the ATP and PPi-dependent H+ transport in the tonoplast were activated by, again in this order, 13%, 13% and 9%, in accordance with the decrease in [Pi]c. We propose that the change in [Pi]c is one of the regulators of photosynthesis-mediated activation of the H+-pumps in the plasma membrane and the tonoplast in C. corallina upon illumination.

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Abbreviations

Em :

membrane potential

[Pi]c :

cytoplasmic Pi level

[Pi]v :

vacuolar Pi level

Rm :

membrane resistance

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Author notes

  1. Kazuhiko Takeshige

    Present address: Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Asahi-machi 3-6-6, Machida, 194, Tokyo, Japan

  2. Masashi Tazawa

    Present address: Department of Applied Physics and Chemistry, Fukui Institute of Technology, Gakuen, 910, Fukui, Japan

  3. Tetsuro Mimura

    Present address: Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, 678-12, Hyogo, Japan

Authors and Affiliations

  1. Department of Botany, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Kazuhiko Takeshige, Masashi Tazawa & Tetsuro Mimura

  2. National Institute for Environmental Studies, 305, Tsukuba, Ibaraki, Japan

    Fumiyuki Mitsumori

Authors
  1. Kazuhiko Takeshige
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  2. Fumiyuki Mitsumori
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  3. Masashi Tazawa
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  4. Tetsuro Mimura
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Additional information

The authors are grateful to Professor T. Nagata (University of Tokyo) and Dr. K. Sakano (National Institute of Agrobiological Resources) for allowing us to carry out a part of our study in their laboratories. We also thank to Professor T. Shimmen (Himeji Institute of Technology) for his helpful discussion and critical reading of the manuscript. One of authors, T.M. especially thanks Drs. U. Heber (Universität Würzburg, FRG) and M. Stitt (Universität Bayreuth, FRG) for their stimulating suggestions related to the present study. This work was supported by a Grant-in-Aid for Science Research from the Ministry of Education, Science and Culture,Japan.

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Takeshige, K., Mitsumori, F., Tazawa, M. et al. Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina . Planta 186, 466–472 (1992). https://doi.org/10.1007/BF00195329

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