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Plant regeneration through somatic embryogenesis from suspension culture-derived protoplasts of Paspalum scrobiculatum L.

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Abstract

Protoplasts were released from embryogenic suspension culture of Paspalum scrobiculatum and cultured in either liquid or semisolid KM medium supplemented with 2,4-D in the dark at 24°C with or without a feeder layer. Cell wall formation was observed in 75% of the plated protoplasts. Microcolonies developed after 10 d of culture, which in turn formed callus upon transfer to M-2 medium (Nayak and Sen, 1989). The highest plating effeciency (ca 7%) was obtained in thin-layer liquid culture. The macrocalli formed somatic embryos which regenerated to plantlets. The plantlets were grown to flowering plants upon transfer to soil.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

FDA:

Fluoresceine diacetate

MES:

2-(N-Morpholino) ethanesulfonic acid

MS:

Murashige & Skoog medium (1962)

References

  • Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technol 4: 1087–1090

    Google Scholar 

  • Dalton SJ (1988) Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb. (tall fescue) and Lolium perenne L. (perennial ryegrass). J. Plant Physiol 132: 170–175

    Google Scholar 

  • Datta SK, Peterhans A, Datta K, Potrykus I (1990) Genetically engineered fertile indica rice recovered from protoplasts. Bio/Technol 8: 736–740

    Google Scholar 

  • Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC, Caplan AB (1990) Transient gene expression in intact and organised rice tissues. The Plant Cell 2: 591–602

    Google Scholar 

  • Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technol 8: 833–839

    Google Scholar 

  • Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603–618

    Google Scholar 

  • Harris R, Wright M, Byrne M, Varnum J, Brightwell B, Schubert K (1988) Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.). Plant Cell Rep 7: 337–340

    Google Scholar 

  • Heyser JW (1984) Callus and shoot regeneration from protoplasts of proso millet (Panicum miliaceum L.) Z Pflanzenphysiol 113: 293–299

    Google Scholar 

  • Horn ME, Conger BV, Harms CT (1988) Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.). Plant Cell Rep 7: 371–374

    Google Scholar 

  • Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at very low population density in liquid media. Planta 126: 105–110

    Google Scholar 

  • Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206: 408–413

    Google Scholar 

  • Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76: 887–890

    Google Scholar 

  • Lee L, Schroll RE, Grimes HD, Hodges TK (1989) Plant regeneration from indica rice (Oryza sativa L.) protoplasts. Planta 178: 325–333

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    CAS  Google Scholar 

  • Nayak P, Sen SK (1989) Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum. Plant Cell Rep 8: 296–299

    Google Scholar 

  • Nayak P, Sen SK (1990) Plant regeneration from protoplasts of indica rice. In: 4th Annual Meeting of The Rockefeller Foundations International Programme on Rice Biotechnology, IRRI, Manila May, 1990

    Google Scholar 

  • Potrykus I (1990) Gene transfer to cereals: An assessment. Bio/Technol 8: 532–542

    Google Scholar 

  • Prioli LM, Söndahl MR (1989) Plant regeneration and recovery of fertile plants from protoplasts of maize (Zea mays L.) Bio/Technol 7: 589–594

    Google Scholar 

  • Raineri DM, Bottino P, Gordon MP, Nester EW (1990) Agrobacterium — mediated transformation of rice (Oryza sativa L.). Bio/Technol 8: 33–38

    Google Scholar 

  • Rhodes CA, Lowe KS, Ruby KL (1988) Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Bio/Technol 6: 56–60

    Google Scholar 

  • Shillito RD, Carswell GK, Johnson CM, DiMaio JJ, Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technol 7: 581–587

    Google Scholar 

  • Srinivasan C, Vasil IK (1986) Plant regeneration from protoplasts of sugarcane. J Plant Physiol 126: 41–48

    Google Scholar 

  • Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technol 8: 429–434

    Google Scholar 

  • Wei Z, Xu Z (1990) Regeneration of fertile plants from embryogenic suspension culture protoplasts of Sorghum vulgare. Plant Cell Rep 9: 51–53

    Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47: 189–191

    Google Scholar 

  • Yamada Y, Yang ZQ, Tang DT (1986) Plant regeneration from protoplast derived callus of rice (Oryza sativa L.). Plant Cell Rep 4: 85–88

    Google Scholar 

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Authors and Affiliations

  1. Plant Molecular & Cellular Genetics, Bose Institute Centenary Building, P-1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India

    Pritilata Nayak & S. K. Sen

Authors
  1. Pritilata Nayak
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  2. S. K. Sen
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Communicated by C. T. Harms

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Nayak, P., Sen, S.K. Plant regeneration through somatic embryogenesis from suspension culture-derived protoplasts of Paspalum scrobiculatum L.. Plant Cell Reports 10, 362–365 (1991). https://doi.org/10.1007/BF00193160

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  • DOI: https://doi.org/10.1007/BF00193160

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