Abstract
14C-Labelled CO2 fed to young Norway spruce (Picea abies [L.] Karst.) twigs was rapidly incorporated into kaempferol 3-O-glucoside (astragalin) of the needles. The patterns of the time course of total (per needle weight) and specific radioactivity (per amount of compound) of soluble and insoluble (cell wall-bound) astragalin indicate its transport from a soluble pool within the protoplast to an extraprotoplastic cell wall-bound pool within the needle. This conclusion is supported by measurements of the distribution of radioactivity between the aglycone (kaempferol) and the sugar part (glucose) of the molecule after various chase periods as well as by control experiments to determine the localization of [14C]astragalin in the cell wall preparations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alcubilla, M. (1970) Pilzhemmstoffe in der Fichtenrinde. Landwirtschaftl. Forsch. 25/I, 96–101
Barz, W., Köster, J., Weltring, K.-M., Strack, D. (1985) Recent advances in the metabolism of phenolic compounds in plants and animals. Annu. Proc. Phytochem. Soc. 25, 307–347
Brandner, G. (1962) Markierungsversuche mit radioaktiven Kohlenstoffverbindungen und anderen Untersuchungen zur Bildung von Isoflavonen in Pflanzen. Dissertation, University of Freiburg, FRG
Brandner, G., Grisebach, H. (1962) Einbau des 2′,4,4′,6′-Tetrahydroxy-chalkon-2′-glucoside-[β-14C] in Isoflavone. Experientia 18, 400–403
Dieterman, L.J., Wender, S.H., Chorney, W., Skok, S.H. (1969) Incorporation of 14C-quercetin into an isopropyl alcohol- and acid-insoluble fraction of tobacco. Phytochemistry 8, 2321–2323
Dittrich, P., Kandler, O. (1971) Einfluß der Jahreszeit auf Bildung und Umsatz von Phenolkörpern in der Fichte (Picea abies [L.] Karst.). Ber. Dtsch. Bot. Ges. 84, 465–472
Enoki, A., Yaku, F., Koshijima, T. (1983) Synthesis of LCC model compounds and their chemical and enzymatic stabilities. Holzforschung 37, 135–141
Friend, J. (1981) Plant phenolics, lignification and plant disease. In: Progress in phytochemistry, vol. 7, pp. 220–224, Harborne, J.B., Swain, T., eds. Pergamon Press, New York
Fry, S.C. (1984) Incorporation of [14C]cinnamate into hydrolaseresistant components of the primary cell wall of spinach. Phytochemistry 23, 59–64
Fry, S.C. (1986) Cross-linking of matrix polymers in the growing cell walls of angiosperms. Annu. Rev. Plant Physiol. 37, 165–186
Fry, S.C. (1987) Intracellular feruloylation of pectic polysaccharides. Planta 171, 205–211
Ibrahim, R.K., De Luca, V., Khouri, H., Latchinian, L., Brisson, L., Charest, P.M. (1987) Enzymology and compartmentation of polymethylated flavonol glucosides in Chrysosplenium americanum. Phytochemistry 26, 1237–1245
Jahnke, S. (1978) Zur Messung von Photosyntheseraten intakter Blätter. Eine Methode zur Applikation von 11CO2 und 14CO2 unter kontrollierten Bedingungen im Hinblick auf quantitative Untersuchungen des Phloemtransports. Diplomarbeit, University of Köln, FRG
Jahnke, S., Stöcklin, G., Willenbrink, J. (1981) Translocation profiles of 11C-assimilates in the petiole of Marsilea quadrifolia L. Planta 153, 56–63
Harris, P.J., Hartley, R.D. (1980) Phenolic constituents of the cell walls of monocotyledons. Biochem. Syst. Ecol. 8, 153–160
Leary, G.J. (1980) Quinone methides and the structure of lignin. Wood Sci. Technol. 14, 21–34
Matile, P. (1984) Das toxische Kompartiment der Pflanzenzelle. Naturwissenschaften 71, 18–24
Meyer, E. (1983) Peroxidase catalyzed incorporation into polymers as a major pathway of hordenine metabolism in barley cell suspension cultures. Phytochemistry 22, 1381–1385
Osswald, W.F., Zieboll, S., Schütz, W., Firl, J., Elstner, E.F. (1987) p-Hydroxyacetophenone a fungitoxic compound in spruce needles. Z. Pflanzenkr. Pflanzenschutz 94, 572–577
Porter, L.J., Foo, L.Y., Furneaux, R.H. (1985) Isolation of three naturally occurring O-β-glucopyranosides of procyanidin polymers. Phytochemistry 24, 567–569
Stafford, H.A. (1988) Proanthocyanidin and the lignin connection. Phytochemistry 27, 1–6
Strack, D., Heilemann, J., Klinkott, E.-S., Wray, V. (1988a) Cell wall-bound phenolics from Norway Spruce (Picea abies) needles. Z. Naturforsch. 43c, 37–41
Strack, D., Heilemann, J., Mömken, M., Wray, V. (1988b) Cell wall-conjugated phenolics from Coniferae leaves. Phytochemistry 27, 3517–3521
Strack, D., Heilemann, J., Wray, V., Dirks, H. (1989) Structures and accumulation patterns of soluble and insoluble phenolics from Norway spruce needles. Phytochemistry 28, 2071–2078
Swain, T. (1977) Secondary compounds as protective agents. Annu. Rev. Plant Physiol. 28, 479–501
Wagner, G.J. (1982) Compartmentation in plant cells: The role of the vacuole. Recent Adv. Phytochem. 12, 1–45
Author information
Authors and Affiliations
Additional information
This work was supported by the Deutsche Forschungsgemeinschaft (Schwerpunktprogramm “Physiologie der Bäume”) and by the Fonds der Chemischen Industrie. We express our special thanks to J. Willenbrink and T. Schatten (Botanisches Institut, Köln, FRG) for advice and help in the 14CO2-application experiments. We also thank H. Grisebach (Biologisches Institut II, Freiburg, FRG) for drawing our attention to the work of G. Brandner.
Rights and permissions
About this article
Cite this article
Heilemann, J., Strack, D. Incorporation of kaempferol 3-O-glucoside into the cell walls of Norway spruce needles. Planta 181, 599–603 (1990). https://doi.org/10.1007/BF00193016
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00193016