Abstract
A skim-milk fraction and a whey-protein concentrate (WPC) fraction were prepared from the cows that had been immunized with E. coli isolated from the mouse intestine. The antibacterial effect of these fractions against E. coli was examined. They contained antibody with a high affinity for E. coli strain 48, a representative strain in the mouse intestine, which is composed of a large amount of IgG and smaller amouns of IgA and IgM. Although these fractions showed no bactericidal or bacteriostatic activity against E. coli 48 directly in vitro, they exhibited strong agglutination and opsonization activities against the bacteria in vitro. The bacteria opsonized with the WPC fraction were taken up more effectively by liver macrophages in vivo, compared with unopsonized E. coli, after an intravenous injection into mice. Oral administration of the skim-milk fraction to mice significantly reduced the susceptibility to the lethal toxicity of 5-fluorouracil (5 FU). The increase in the population levels of E. coli in the intestinal tract after administration of 5 FU was inhibited by oral administration of the skimmilk fraction. These results strongly suggest that specific antibody may be effective in the prophylaxis against the indigenous infection with gram-negative bacteria such as E. coli after a period of chemotherapy in cancer patients.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bellamy JEC, Nielsen NO (1974) Immune-mediated emigration of neutrophils into the lumen of the small intestine. Infect Immun 9:615–619
Berg RD, Garlington AW (1979) Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun 23:403–411
Chaicumpa W, Rowley D (1973) Mechanisms of antibacterial immunity against Vibrio cholerae in the intestinal tracts of baby mice. J Infect Dis 128:56–61
Ebina T, Sato A, Umezu K, Ishida N, Ohyama S, Oizumi A, Aikawa K, Katagiri K, Katsushima N, Imai A, Kitaoka S, Suzuki H, Konno T (1985) Prevention of rotavirus infection by oral administration of cow colostrum containing anti-humanrotavirus antibody. Med Microbiol Immunol 174:177–185
Glucksberg H, Cheever MA, Farewell VT, Freter A, Sale WE, Thomas ED (1981) High-dose combination chemotherapy for acute nonlymphoblastic leukemia in adults. Cancer 48:1073–1081
Hilpert H, Gerber H, Amster H, Pahud JJ, Ballabriga A, Arcalis L, Farriaux F, de Peyer E, Nussle D (1977) Bovine milk immunoglobulins (Ig), their possible utilization in industrially prepared infant's milk formulae. In: Hambraeus L, Hanson LA, McFarlane H (eds) Food and Immunology. Swedish Nutrition Foundation, Stockholm, pp 182–196
Hilpert H, Brussow H, Mietens C, Sidoti J, Lerner L, Werchau H (1987) Use of bovine milk concentrate containing antibody to rotavirus to treat rotavirus gastroenteritis in infants. J Infect Dis 156:158–166
Keating MJ, Smith TL, McCredie KB, Body GP, Hersh EM, Gutterman JU, Gehan E, Freireich EJ (1981) A four-year experience with anthracycline, cytosine arabinoside, vincristine and prednisone combination chemotherapy in 325 adults with acute leukopenia. Cancer 47:2779–2788
Kreger BE, Craven DE, CarLing PC, McCabe WR (1980) Gram-negative bacteremia. III. Reassessment of etiology, epidemiology and ecology in 612 patients. Am J Med 68:332–343
Law BA, Reiter B (1977) The isolation and bacteriostatic properties of lactoferrin from bovine milk whey. J Dairy Res 44:595–599
Miake S, Nomoto K, Yokokura T, Yoshikai Y, Mutai M, Nomoto K (1985) Protective effect of Lactobacillus casei on Pseudomonas aeruginosa infection in mice. Infect Immun 48:480–485
Nomoto K, Miake S, Hashimoto S, Yokokura T, Mutai M, Nomoto K (1985) Augmentation of host resistance to Listeria monocytogenes infection by Lactobacillus casei. J Clin Lab Immunol 17:91–97
Nomoto K, Yokokura T, Yoshikai Y, Mitsuyama M, Nomoto K (1991) Induction of lethal infection with indigenous Escherichia coli in mice by fluorouracil. J Can Microbiol 37:244–247
Pizzo PA (1989) Considerations for the prevention of infectious complications in patients with cancer. Rev Infect Dis 11:1551–1563
Reiter B, Brock JH (1975) Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. Immunology 28:71–82
Tancrède CH, Andremont AO (1985) Bacterial translocation and gram-negative bacteremia in patients with hematological malignancies. J Infect Dis 152:99–103
Vakil JR, Chandan RC, Parray RM, Shahani KM (1969) Susceptibility of several microorganisms to milk lysozymes. J Dairy Res 52:1192–1197
Verhoef J, Rozenberg-Arska M, Dekker A (1989) Prevention of infection in the neutropenic patients. Rev Infect Dis 11:1545–1550
Wells CL, Podzorski RP, Peterson PK, Ramsay NK, Simmons RL, Rhames FS (1984) Incidence of trimethoprim-sulfamethoxazole-resistant Enterobacteriaceae among transplant recipients. J Infect Dis 150:699–706
Wells CL, Ferrieri P, Weisdorf DJ, Rhame FS (1987) The importance of surveillance stool culture during periods of severe neutropenia. Infect Control 8:317–319
Wells CL, Maddaus MA, Simmons RL (1988) Proposed mechanisms for the translocation of intestinal bacteria. Rev Infect Dis 10:958–979
Wernet P, Breu H, Knop J, Rowley D (1971) Antibacterial action of specific IgA and transport of IgM, IgA and IgG from serum into the small intestine. J Infect Dis 124:223–226
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nomoto, K., Matsuoka, Y., Hayakawa, K. et al. Antibacterial effect of bovine milk antibody against Escherichia coli in a mouse indigenous infection model. Med Microbiol Immunol 181, 87–98 (1992). https://doi.org/10.1007/BF00189427
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00189427