Summary
The electropheretic karyotype for the imperfect filamentous fungus Tolypocladium inflatum and for six related strains is presented. Pulsed-field gel electrophoresis was used with improved separation conditions to separate DNA from 6.6 Mb to 1.05 Mb in size. Using probes encoding rRNA or the β-tubulin gene from T.iinflatum the corresponding genes were detected on designated chromosomes. In addition, two recombinant lambda clones, carrying T. inflatum chromosomal DNA, were used as chromosome-specific probes. Although all strains investigated are very similar in their morphology, significant chromosome-lengt polymorphisms were detected, allowing easy strain differentiation. The polymorphism was confirmed using an rRNA probe for genomic mapping. All strains contain a homologous “minichromosome” of 1.05 Mb. Finally, the resolution of very large DNA enabled us to separate a 6.6-Mb DNA band that specifically hybridizes with mitochondrial gene probes. The electrophoretic karyotyping presented here may be regarded as a reliable molecular tool to differentiate morphologically very similar filamentous fungi.
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Stinberg, N., Walz, M., Schörgendorfer, K. et al. Electrophoretic karyotyping from Tolypocladium inflatum and six related strains allows differentiation of morpologically similar species. Appl Microbiol Biotechnol 37, 485–489 (1992). https://doi.org/10.1007/BF00180974
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DOI: https://doi.org/10.1007/BF00180974