Summary
Total saliva and secretions from parotid and submandibular glands of patients with carcinomas in the oral cavity, oropharynx or larynx and a control group of healthy individuals were analyzed for concentrations of glycosidically bound sugars and free N-acetylneuraminic acid as well as for sialidase activity. When compared to the data obtained for normal donors, the relative amounts of the individual monosaccharides fucose, galactose, mannose, N-acetylgalactosamine, and N-acetylglucosamine as components of glycoconjugates showed variable differences to the group of tumor patients depending on the type of secretion and the location of the tumor. The percentage of glycosidically linked N-acetylneuraminic acid, however, was always higher for the healthy donors. A significant difference was found in the amount of free sialic acid, with the exception of submandibular gland secretion from a patient with an oropharyngeal carcinoma, and sialidase activity which were increased for tumor patients, independent of the type of secretion and the location of the tumor. From these results it is concluded that free sialic acid and sialidase activity may be considered as markers for carcinomas in the upper aerodigestive tract.
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References
Abe K, Nitta H (1984) Amino acid composition of salivary protein secreted by the parotid glands of rats in response to parasympathomimetic and sympathomimetic drugs. Arch Oral Biol 29:127–130
Baum BJ (1987) Saliva secretion and composition. Front Oral Physiol 6:126–134
Berg W, Gutschker-Gdaniec G, Schauer R (1985) Fluorescent staining of sialidases in polyacrylamide gel electrophoresis and ultrathin-layer isoelectric focusing. Anal Biochem 145:339–342
Bumm P, Schauer R (1982) Zur Bedeutung menschlicher Speichel- und Schleimhautmuzine im Bereich der oberen Luft- und Speisewege. HNO 30:414–419
Dische Z (1962) Color reactions of hexoses. Methods Carbohydr Chem 1:488–494
Froehlich DA, Pangborn RM, Whitaker JR (1987) The effect of oral stimulation on human parotid salivary flow rate and alpha-amylase secretion. Physiol Behav 41:209–217
Kamerling JP, Gerwig GJ, Vliegenthart JFG, Clamp JR (1975) Characterization by gas-liquid chromatography-mass spectrometry and proton-magnetic resonance spectroscopy of pertrimethylsilyl methyl glycosides obtained in methanolysis of glycoproteins and glycopeptides. Biochem J 151:491–495
Kousvelari EE, Banerjee DK, Murty L, Baum BJ (1988) N-Linked protein glycosylation in the rat parotid gland during aging. Mech Ageing Dev 42:173–181
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Maier H, Born IA, Adler D (1986) Eingeschränkte Funktion der großen Kopfspeicheldrüsen. Laryngol Rhinol Otol 65:195–200
Maier H, Born IA, Mall G (1988) Effect of chronic ethanol and nicotine consumption on the function and morphology of the salivary glands. Klin Wochenschr 66 [Suppl 11]:140–150
Marmary Y, Fox PC, Baum BJ (1987) Fluid secretion rates from mouse and rat parotid glands are markedly different following pilocarpine stimulation. Comp Biochem Physiol 88A:307–310
Nieuw Amerongen AV, Oderkerk C, Lange GL de, Bos-Vreugdenhil AP, Roukema PA (1985) Biochemical characteristics and protein composition of murine saliva evoked in vivo by receptor-selective agonists. J Biol Buccale 13:167–178
Potier M, Mameli L, Belisle M, Dallaire L, Melancon SB (1979) Fluorimetric assay of neuraminidase with a sodium-(4methylumbelliferyl-alpha-d-N-acetylneuraminate) substrate. Anal Biochem 94:287–296
Reuter G, Schauer R (1986) Comparison of electron and chemical ionization mass spectrometry of sialic acids. Anal Biochem 157:39–46
Reuter G, Pfeil R, Stoll S, Schauer R, Kamerling JP, Versluis C, Vliegenthart JFG (1983) Identification of new sialic acids derived from glycoprotein of bovine submandibular gland. Eur J Biochem 134:139–143
Schauer R (1982) Chemistry, metabolism, and biological functions of sialic acids. Adv Carbohydr Chem Biochem 40:131–234
Schauer R (1985) Sialic acids and their role as biological masks. Trends Biochem Sci 10:357–360
Schauer R (1987) Analysis of sialic acids. Methods Enzymol 138:132–161
Shukla AK, Schauer R (1982) Analysis of N, O-acylated neuraminic acids by high-performance liquid anion-exchange chromatography. J Chromatogr 244:81–89
Slomiany BL, Liau YH, Zalesna G, Slomiany A (1988) Effect of ethanol on the in vitro sulfation of salivary mucin. Alcohol Clin Exp Res 12:774–779
Takagi A, Slomiany BL, Kosmala M, Slomiany A (1986) Changes in mucus glycoprotein synthesized in rat gastric mucosa exposed to ethanol. Biochim Biophys Acta 884:1–10
Tylenda CA, Ship JA, Fox PC, Baum BJ (1988) Evaluation of submandibular salivary flow rate in different age groups. J Dent Res 67:1225–1228
Warner TG, O'Brien JS (1979) Synthesis of 2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid and detection of skin fibroblast neuraminidase in normal humans and in sialidosis. Biochemistry 18:2783–2787
Watanabe S, Dawes C (1988) The effects of different foods and concentrations of citric acid on the flow rate of whole saliva in man. Arch Oral Biol 1:1–5
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Reuters, G., Struwe, R., Feigel, J. et al. Analysis of carbohydrate composition and sialidase activity in oral secretions of patients with tumors in the upper aerodigestive tract. Eur Arch Otorhinolaryngol 249, 5–11 (1992). https://doi.org/10.1007/BF00175662
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DOI: https://doi.org/10.1007/BF00175662