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Comparative study of nickel toxicity to growth and photosynthesis in nickel-resistant and -sensitive strains of Scenedesmus acutus f. alternans (Chlorophyceae)

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Abstract

Nickel (Ni) toxicity to growth and photosynthesis was studied in four strains of Scenedesmus acutus f. alternans. Effects of Ni dosage and duration of exposure on growth and photosynthesis were strain specific. Large differences in responses of both growth and photosynthesis to Ni were detected between three resistant strains (B4, Cu-Tol, and Ni-Tol) and one sensitive strain (UTEX 72). Growth of UTEX 72 was ≥ 18 times more sensitive to Ni than those of the three resistant strains. The order of Ni dosages (fmol Ni/pg cell dry weight) causing 50% inhibition (D150) of growth rates in the four strains was Ni-Tol (10.5) > B4 (8.19) > Cu-Tol (4.60) > UTEX 72 (0.25). The effect of Ni dosage on photosynthetic rate as percentage of control corresponded to a saturation curve and was a strong function of duration of exposure. The DI50s of photosynthetic rates were ≥3.5 times lower in UTEX 72 than in the three resistant strains, and in all four strains they decreased sharply with the increase in duration of exposure. The order of the four strains in DI50s of photosynthetic rate was B4 (58.2) > Cu-Tol (38.0) > Ni-Tol (28.9) > UTEX 72 (8.24) for 6-h exposure and Ni-Tol (2.88) > Cu-Tol (1.30) > B4 (1.01) > UTEX 72 (0.15) for 24-h exposure. The DI50s of photosynthetic rate for 6-h exposure were higher than those of growth rate in all four strains, and for 24-h exposure they were lower, except in UTEX 72. Thus, the relative Ni sensitivity of growth and photosynthesis of the four strains depends on the duration of exposure. The results of factorial analysis of variance suggested that Ni toxicity to photosynthesis is a consequence of a strong interaction among strain, Ni dosage, and duration of exposure.

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Authors and Affiliations

  1. Department of Botany, University of Toronto, M5S 3B2, Toronto, Ontario, Canada

    X. Jin

  2. Life Science Division, Scarborough College, University of Toronto, 1265 Military Trail, MIC 1A4, Scarborough, Ontario, Canada

    C. Nalewajko

  3. Department of Microbiology, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    D. J. Kushner

  4. Department of Botany, University of Toronto, M5S 3B2, Toronto, Ontario, Canada

    D. J. Kushner

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  2. C. Nalewajko
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  3. D. J. Kushner
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Jin, X., Nalewajko, C. & Kushner, D.J. Comparative study of nickel toxicity to growth and photosynthesis in nickel-resistant and -sensitive strains of Scenedesmus acutus f. alternans (Chlorophyceae). Microb Ecol 31, 103–114 (1996). https://doi.org/10.1007/BF00175079

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  • DOI: https://doi.org/10.1007/BF00175079

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