Summary
The effect of calcium ions on production of extracellular L-glutamate oxidase (GluOx) in liquid fermentation of Streptomyces sp. N1 was investigated. By supplementing a relatively large amount of Ca2+ (15–40 mM) to the medium, the GluOx production was significantly enhanced, although the microbial growth was inhibited to some degree. For the first time, the highest production (i.e. 6.5 U/ml) and productivity (i.e. 0.25 U/ml/h) of GluOx as ever reported (i.e., 1.2 U/g medium after 144 h solid state fermentation and 0.59 U/ml after 96 h liquid fermentation) were achieved with addition of 30 mM Ca2+.
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Li, Q.S., Zhong, J.J. Enhancement of L-glutamate oxidase production in liquid fermentation of Streptomyces sp. by calcium addition. Biotechnol Tech 10, 453–456 (1996). https://doi.org/10.1007/BF00174232
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DOI: https://doi.org/10.1007/BF00174232