Abstract
In cultured bovine adrenal chromaffln cells, charybdotoxin and iberiotoxin (inhibitors of the large-conductance Ca2+-activated K+ channel) as well as apamin (an inhibitor of the small-conductance Ca2+-activated K+ channel), at 1–100 nM, suppressed carbachol-induced 86Rb+ efflux, augmented carbachol-induced 45Ca2+ influx via voltage-dependent Ca2+ channels and catecholamine secretion and had no effect on carbachol-induced 22Na+ influx via nicotinic receptors, a prerequisite for Ca2+ channel activation by carbachol. 45Ca2+ influx caused by high K+ (a direct activation of voltage-dependent Ca2+ channels) was also enhanced by these K+ channel inhibitors, with the concentration-response curves being similar to those for carbachol-induced 45Ca2+ influx. Dendrotoxin and mast cell degranulating peptide (inhibitors of voltage-dependent K+ channels), on the other hand, did not alter carbachol-induced 86Rb+ efflux or 45Ca2+ influx.
These results suggest that the stimulation of nicotinic receptors eventually opens large- and small-conductance Ca2+-activated K+ channels, and that the blockade of these Ca2+-activated K+ channels results in gating of voltage-dependent Ca2+ channels and thereby augments catecholamine secretion from bovine adrenal chromaffln cells.
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Wada, A., Urabe, M., Yuhi, T. et al. Large- and small-conductance Ca2+-activated K+ channels: their role in the nicotinic receptor-mediated catecholamine secretion in bovine adrenal medulla. Naunyn-Schmiedeberg's Arch Pharmacol 352, 545–549 (1995). https://doi.org/10.1007/BF00169389
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DOI: https://doi.org/10.1007/BF00169389