Abstract
The qualitative and quantitative composition of bacterial flora occurring inside the leaf spots of field grown soybeans was studied during the growing seasons (June to October) of 1989 and 1990. As a rule these leaf spots (necrotic lesions with chlorotic haloes) were caused by Pseudomonas syringae pv. glycinea. This pathogenic bacterium was predominantly found during the whole season in the symptomatic leaf tissue. Other species, mainly Erwinia herbicola, were also found in the same habitat. The population sizes of P. s. pv. glycinea increased from the beginning of symptom occurrence until July, stabilized until September, and then decreased a little. In general, the size of saprophytic populations was orders of magnitude lower than that of the pathogenic populations. The number of different bacterial genera per sample increased up to four genera per leaf spot by the end of the season. No significant influence of the occurring saprophytes on the population dynamics of the pathogen in planta could be observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blakeman JP (1982) Phylloplane interactions. In: Mount MS, Lacy GH (eds) Phytopathogenic prokaryotes, vol. 1. Academic Press, New York, pp 307–333
Bradbury JF (1988) Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methodes. Acta Horticulturae 225:27–37
Dickinson CH, Austin B, Goodfellow M (1975) Quantitative and qualitative studies of phylloplane bacteria from Lolium perenne. J Gen Microbiol 91:157–167
Dye DW (1983) Erwinia: The “arnylovora” and “herbicola” groups. In: Fatty PC, Persley GJ (eds) Plant bacterial diseases—A diagnostic guide. Academic Press, Sydney, pp 67–86
Fahy PC, Hayward AC (1983) Media and methods for isolation and diagnostic tests. In: Fahy PC, Persley GJ (eds) Plant bacterial diseases—A diagnostic guide. Academic Press, Sydney, pp 337–375
Gregersen T (1978) Rapid method for distinction of gram-negative from gram-positive bacteria. European J Appl Microbiol Biotechnol 5:123–127
Hugh R, Leifson E (1953) The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66:24–26
King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clinical Medicine 44:301–307.
Kuriger WE, Roane CW (1981) Identification and population dynamics of bacteria in symptomatic oat leaves. Microb Ecol 7:23–28
Leben C (1965) Epiphytic microorganisms in relation to plant disease. Ann Rev Phytopathology 3:209–230
Lelliott RA, Billing E, Hayward AC (1966) A determinative scheme for the fluorescent plant pathogenic Pseudomonads. J Appl Bacteriol 29:470–480
Mew TW, Kennedy BW (1982) Seasonal variation in populations of pathogenic Pseudomonads on soybean leaves. Phytopathology 72:103–105
Mew TW, Vera Cruz CM (1986) Epiphytic colonization of host and non-host plants by phytopathogenic bacteria. In: Fokkema NJ, Van den Heuvel J (eds) Microbiology of the phyllosphere. Cambridge University Press, Cambridge, pp 269–281
Sinclair JB, Shurtleff MC (1975) Compentium of soybean diseases. Am Phytopath Soc Inc St. Paul, Minnesota
Sundin GW, Jones AL, Olson BD (1988) Overwintering and population dynamics of Pseudomonas syringae pv. syringae and P. s. pv. morsprunorum on sweet and sour cherry trees. Can J Plant Pathol 10:281–288
Author information
Authors and Affiliations
Additional information
Send offprint requests to: Dr. Beate Völksch, Friedrich Schiller University Jena, Biological Faculty, Institute of Microbiology, Pbilosophenweg 12, D/0-6900 Jena, Germany.
Rights and permissions
About this article
Cite this article
Völksch, B., Ullrich, M. & Fritsche, W. Identification and population dynamics of bacteria in leaf spots of soybean. Microb Ecol 24, 305–311 (1992). https://doi.org/10.1007/BF00167788
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00167788