Summary
Sympathetic denervation of the rat heart was produced by 6-hydroxydopamine. In denervated ventricles abundant “atrial” granules were observed whereas in controls no such granules were seen. Denervated ventricles and atria showed a very high content of atrial natriuretic peptide-like immunoreactivity (> 10 times higher than in control animals). We suggest that the sympathetic nervous system exerts a repressive effect upon synthesis of atrial natriuretic peptide-like material and upon the formation of atrial natriuretic peptide-storing granules in the ventricular myocardium.
This is a preview of subscription content, log in via an institution to check access.
References
Albino-Teixeira A, Azevedo I, Osswald W (1988) Prevention by purines of the structural changes caused at the extraneuronal level by surgical and chemical denervation. Br J Pharmacol 95:785P
Anderson JV, Christofides ND, Vinas P, Wharton J, Varndell IM, Polak JM, Bloom SR (1986) Radioimmunoassay of alpha rat atrial natriuretic peptide. Neuropeptides 7:159–173
Azevedo I, Osswald W (1986) Throphic role of the sympathetic innervation. J Pharmacol (Paris) 17 (suppl 11):30–43
Azevedo I, Castro-Tavares J, Garrett J (1981) Ultrastructural changes in blood vessels of perinephritic hypertensive dogs. Blood Vessels 18:110–119
Bloch KID, Seidman JC, Naftilan JD, Fallon JT, Seidman CE (1986) Neonatal atria and ventricles secrete atrial natriuretic factor via tissue-specific secretory pathways. Cell 47:695–702
Branco D, Albino-Teixeira A, Azevedo I, Osswald W (1984) Structural and functional alterations caused at the extraneuronal level by sympathetic denervation of blood vessels. NaunynSchmiedeberg's Arch Pharmacol 326:302–312
Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, Minli S, Chapeau C, Garcia R, Hamet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Hystochemistry 80:113–127
Chidsey AC, Kaiser GA, Sonnenblick EH, Spann JF, Braunwald E (1964) Cardiac norepinephrine stores in experimental heart failure in the dog. J Clin Invest 43:2386–2393
Currie MG, Newman WH (1986) Evidence for alpha-1 adrenergic receptor modulation of atriopeptin release from the isolated rat heart. Biochem Biophys Res Commun 137:94–100
De Champlain J, Malmfors T, Olson L, Sachs CA (1970) Ontogenesis of peripheral adrenergic neurons in the rat pre- and postnatal observations. Acta Physiol Scand 80:276–288
Edwards BS, Ackermann DM, Lee ME, Reeder GS, Wold LE, Burnett JC (1988) Identification of atrial natriuretic factor within ventricular tissue in hamsters and humans with congestive heart failure. J Clin Invest 81:82–86
Gardner DG, Deschepper CF, Ganong WF, Hane S, Fiddes J, Baxter JD, Lewicki J (1986) Extra-atria expression of the gene for atrial natriuretic factor. Proc Natl Acad Sci USA 83:6697–6701
Hassall CJS, Wharton J, Gulbenkian S, Anderson JV, Frater J, Bailey DJ, Merighi A, Bloom SR, Polak JM, Burnstock G (1988) Ventricular and atrial myocytes of newborn rats synthesise and secrete atrial natriuretic peptide in culture: Lightand electron microscopical localisation and chromatographic examination of stored and secreted molecular forms. Cell Tissue Res 251:161–169
Miyata A, Kangawa K, Toshimori T, Hatoh T, Matsuo H (1985) Molecular forms of atrial natriuretic polypeptides in mammalian tissues and plasma. Biochem Biophys Res Commun 129:248–255
Petterson A, Ricksten S-E, Towle AC, Hedner J, Hedner T (1985) Effect of blood volume expansion and sympathetic denervation on plasma levels of atrial natriuretic factor (ANF) in the rat. Acta Physiol Scand 124:309–311
Sarmento A, Soares-da-Silva P, Albino-Teixeira A, Azevedo I (1987) Effects of denervation induced by 6-hydroxydopamine on cell nucleus activity of atrial and cardiac cells of the dog. J Anton Pharmacol 7:119–126
Tranzer JP, Thoenen H (1968) An electron microscopic study of selective, acute degeneration of sympathetic nerve terminals after administration of 6-hydroxydopamine. Experientia (Basel) 24:155–156
Wharton J, Anderson RH, Springall D, Power RF, Rose M, Smith A, Espejo R, Khaghani A, Wallwork J, Yakub MH, Polak JM (1988) Localization of atrial natriuretic peptide immunoreactivity in the ventricular myocardium and conduction system of the human fetal and adult heart. Br Heart J 60:267–274
Author information
Authors and Affiliations
Additional information
Send offprint requests to A. Albino-Teixeira at the above address
Rights and permissions
About this article
Cite this article
Albino-Teixeira, A., Polonia, J.J. & Azevedo, I. Sympathetic denervation causes atrial natriuretic peptide-storing granules to appear in the ventricular myocardium of the rat. Naunyn-Schmiedeberg's Arch Pharmacol 342, 241–244 (1990). https://doi.org/10.1007/BF00166971
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00166971