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Microencapsulation of yeast cells in the calcium alginate membrane

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Biotechnology Techniques

Summary

Cells of Saccharomyces cerevisiae (ATCC 24858) were encapsulated in the calcium alginate membrane and cultured. Swelling of the capsule was prevented by adding 0.2 g CaCl2 to 1 L growth medium. The dry cell concentration based on the inner volume of the capsule reached 309 g/L, which was much higher than could be obtained by cell entrapment. All the cells remained inside the capsule during the cultivation. The flux of CO2 through the capsule membrane increased approximately twice by adding a nonionic surfactant to the CaCl2 solution during the step of capsule formation.

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References

  • Chang, T. M. S. (1972) Artificial Cells, Charles C. Thomas Pub., Springfield, Illinois.

    Google Scholar 

  • Cheetham, P. S. J., K.W. Blunt and C. Bucke (1979) Biotechnol. Bioeng., 21, 2155–2168.

    Google Scholar 

  • Kierstan, M. and C. Bucke (1979) Biotechnol Bioeng., 19, 387–397.

    Google Scholar 

  • Klein, J. and F. Wagner (1979) in “Characterization of Immobilized Biocatalysts”, K. Buchholz (ed.), Verlag Chemie, Germany, 265–335.

    Google Scholar 

  • Knazek, R.A., P. M. Gullino, P. O. Kohler and R. L. Dedrick (1972) Science, 178, 65–67.

    Google Scholar 

  • Lee, C. W. and H. N. Chang (1987) Biotechnol. Bioeng., 29, 1105–1112.

    Google Scholar 

  • Lim, F. and A. M. Sun (1980) Science, 210, 908–910.

    Google Scholar 

  • Nigam, S. C., I. F. Tsao, A. Sakoda and H. Y. Wang (1988) Biotechnol. Techniques, 2, 271–276.

    Google Scholar 

  • Reuveny, S. A. Mizrahi, M. Kotler and A. Freeman (1983) Biotechnol. Bioeng., 25, 469–480.

    Google Scholar 

  • Tanaka, H., M. Matsumura and I. A. Veliky (1984) Biotechnol. Bioeng., 26,53–58.

    Google Scholar 

  • Tyagi, R. D. and T. K. Ghose (1982) Biotechnol., Bioeng., 24, 781–795.

    Google Scholar 

  • van Wezel, A. L. (1967) Nature, 216, 64–65.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chemical Engineering, Kyungpook National University, 702-701, Taegu, Korea

    Soo Hwan Cheong & Joong Kon Park

  2. BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 305-701, Taejon, Korea

    Beom Soo Kim & Ho Nam Chang

Authors
  1. Soo Hwan Cheong
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  2. Joong Kon Park
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  3. Beom Soo Kim
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  4. Ho Nam Chang
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Cheong, S.H., Park, J.K., Kim, B.S. et al. Microencapsulation of yeast cells in the calcium alginate membrane. Biotechnol Tech 7, 879–884 (1993). https://doi.org/10.1007/BF00156366

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  • DOI: https://doi.org/10.1007/BF00156366

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