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Simultaneous production and partition of chitinase during growth of Serratia marcescens in an aqueous two-phase system

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Biotechnology Techniques

Summary

Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2% (w/v) PEG 20000 and 5% (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymer-free reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one.

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Author notes

  1. Jyh-Ping Chen

    Present address: Department of Chemical Engineering, Chang Gung College of Medicine and Technology, 333, Kwei-San, Tao-Yuan, Taiwan, R.O.C.

Authors and Affiliations

  1. Department of Chemical Engineering, National Cheng Kung University, 701, Tainan, Taiwan, R.O.C.

    Jyh-Ping Chen & Maw-Shyan Lee

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  1. Jyh-Ping Chen
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  2. Maw-Shyan Lee
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Chen, JP., Lee, MS. Simultaneous production and partition of chitinase during growth of Serratia marcescens in an aqueous two-phase system. Biotechnol Tech 8, 783–788 (1994). https://doi.org/10.1007/BF00152884

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