Abstract
When the outer membrane proteins (OMP) of Bacteroides fragilis ATCC 23745 grown under seven different culture conditions were analysed by SDS-PAGE, five of the seven preparations showed similar protein profiles. When the three different OMP preparations were subsequently analysed by Western blotting, that from B. fragilis grown in brain heart infusion broth supplemented with haemin and menadione alone showed more immunodominant bands than those grown with additional cholesterol and methionine or cholesterol and cysteine. Only the former showed immunodominant proteins around 45.6 kDa and 66.2 kDa throughout the course of infection in mice. A protein corresponding to 97.4 kDa was found under all culture conditions. The results emphasise the importance of culture conditions in the expression of immunologically reactive antigens and also the possible use of such antigens in the serodiagnosis of B. fragilis infection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Danielsson, D., Lambe, D. W. & Persson, S. 1974 Immune response to anaerobic infections. In Anaerobic Bacteria: Role in Disease, ed Barlows, A. pp. 173–191. Springfield, IL: Charles C. Thomas Press.
Elhag, K.M. & Alkarmi, T.O. 1991 A study of the antigenic composition of the fragilis group of Bacteroides. Journal of Medical Microbiology 35, 118–122.
Kasper, D.L. & Seiler, M.W. 1975 Immunological characterization of outermembrane complex of Bacteroides fragilis ssp fragilis. Journal of Infectious Diseases 132, 440–450.
Kotarski, S.F. & Salyer's, A.A. 1984 Isolation and characterization of outer membrane of Bacteroides fragilis grown on different carbohydrates. Journal of Bacteriology 158, 102–109.
Laemmli, U.K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Lindberg, A.A., Berthold, P., Nord, C.E. & Weintraub, A. 1979 Encapsulated strains of Bacteroides fragilis in clinical specimens. Medical Microbiology and Immunology 167, 29–36.
Loeb, M.R. & Smith, D.H. 1982. Human antibody response to individual outer membrane proteins of Haemophilus influenzae type-b. Infection and Immunity 37, 1032–1036.
Schnaitman, C.A. 1974. Outer membrane protein of Escherichia coli. IV. Differences in outer membrane proteins due to strain and cultural differences. Journal of Bacteriology 118, 454–464.
Towbin, H.J., Staehelin, J. & Gordon, J. 1979 Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76, 4350–4354.
Additional information
T. Khanna is and D. Panigrahi was with the Department of Medical Microbiology and N.K. Ganguly and S. Majumdar are with the Department of Experimental Medicine, at the Postgraduate Institute of Medical Education and Research, Chandigarh, India. D. Panigrahi is now with Kuwait University, Faculty of Alled Health Science, P.O. Box 31470 Sulaibikhat, 90805, Kuwait
Rights and permissions
About this article
Cite this article
Khanna, T., Panigrahi, D., Ganguly, N.K. et al. Recognition of immunologically important antigens in Bacteroides fragilis infection —an experimental study in mice. World J Microbiol Biotechnol 10, 436–438 (1994). https://doi.org/10.1007/BF00144468
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00144468