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Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

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Abstract

The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlordiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

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Abbreviations

BHT:

butylated hydroxytoluene

DDT:

dichlordiphenyltrichloroethane

LY:

Lucifer Yellow

PB:

phenobarbital

TPA:

12-O-tetradecanoylphorbol-13-acetate

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Authors and Affiliations

  1. Laboratory of Carcinogen Screening Methods, All-Union Cancer Research Center of the AMS USSR, Moscow, USSR

    Irene V. Budunova & Gennady A. Belitsky

  2. Department of Mathematical Methods in Biology Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow, USSR

    Leonid A. Mittelman

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  1. Irene V. Budunova
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  2. Leonid A. Mittelman
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  3. Gennady A. Belitsky
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Budunova, I.V., Mittelman, L.A. & Belitsky, G.A. Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow. Cell Biol Toxicol 5, 77–89 (1989). https://doi.org/10.1007/BF00141066

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