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Examining the relationship between secondary structure and antibody recognition in immunopeptides from foot-and-mouth disease virus

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Summary

The conformation of a peptide that represents antigenic site A of foot-and-mouth disease virus strain C-S8c1 (residues 136–156 of VP1; YTASARGDLAHLTTTHARHLP) has been studied by circular dichroism and compared with three analogs that reproduce amino acid substitutions at position 146 (His→Arg, Gln or Asp) which affect antibody recognition. Four other peptides, incorporating replacements at position 147 predicted to maintain (Leu→Ile, Nle and Ala) or disrupt (Leu→Gly) helical structure at this site, have also been studied. In aqueous solution or in 4 M urea, the spectra of all eight peptides were typical of aperiodic conformation and independent of concentration or pH. However, upon addition of solvents such as methanol or hexafluoroisopropanol, spectral patterns evidenced significant levels (ca. 50%) of helical structure. The single residue substitutions at positions 146 and 147 caused minor to significant variations in the calculated amount of α-helix of the peptides. An attempt to relate these changes in helical content to the antigenic behaviour of the peptides towards five monoclonal antibodies elicited with virus and mapping at site A could not find any straightforward correspondence between the two sets of results. The parent peptide and its His146→Arg analog were also analyzed by circular dichroism in the presence of the Fab fragment of SD6, a monoclonal antibody mapping at site A and much less reactive with viruses carrying the referred mutation. Although a peptide-antibody interaction was evident from spectral changes, careful inspection of the difference spectra (peptide-Fab minus Fab) of both peptides failed to detect any significant distinction between them that could be attributed to their different immunoreactivity. While these findings do not necessarily conflict with previous reports that the interaction of antigenic site A with antibodies is mediated to some extent by the adoption of a helix structure, they suggest that, at least for C-serotype viruses, other structural features in addition to a helical conformation are critically involved in antigenic recognition.

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Author notes

  1. Isabel S. Novella

    Present address: Department of Biology, University of California at San Diego, 92093, La Jolla, CA, U.S.A.

Authors and Affiliations

  1. Department of Organic Chemistry, University of Barcelona, Martí i Franquès 1-11, E-08028, Barcelona, Spain

    Xavier Roig, Isabel S. Novella, Ernest Giralt & David Andreu

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  1. Xavier Roig
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  2. Isabel S. Novella
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  3. Ernest Giralt
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  4. David Andreu
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Roig, X., Novella, I.S., Giralt, E. et al. Examining the relationship between secondary structure and antibody recognition in immunopeptides from foot-and-mouth disease virus. Lett Pept Sci 1, 39–49 (1994). https://doi.org/10.1007/BF00132761

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  • DOI: https://doi.org/10.1007/BF00132761

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