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The regulation of cyclic AMP production and the role of cyclic AMP in B16 melanoma cells of differing metastatic potential

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The nature of the relationship between agonist-stimulated cyclic AMP production and metastatic potential was examined in detail for four B16 melanoma cell lines of varying metastatic potential. Highly metastatic cells (B16 F10C1) appeared to differ from cells of low metastatic potential (B16 F1C29) in the degree to which cyclic AMP production in intact cells was stimulated by protein kinase C activation. No significant difference was found in the adenylate cyclase enzyme activities of the broken cells, irrespective of the agonist used, or in the distribution of cyclic AMP between the intracellular and extracellular compartment. Although B16 F1, F10 and F10C1 cells all produced equally pigmented tumors in vivo, the cells differed in their melanogenic response to cyclic AMP elevating agents in vitro: the least metastatic cells produced least agonist-induced cyclic AMP but this induced greatest tyrosinase activation and melanin production in vitro; conversely, the more metastatic cells produced more cyclic AMP but less tyrosinase activation and melanin production in response to agonist stimulation. Thus, agonist-stimulated cyclic AMP production does not appear to be coupled to the differentiated function of melanogenesis for highly metastatic B16 melanoma cells.

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References

  1. Agarwal K. C., and Parks, R. E., 1983, Forskolin: an antimetastatic agent. International Journal of Cancer, 32, 801–804.

    Google Scholar 

  2. Bell, J. D., Buxton, I. L. O., and Brunton, L. L., 1983, Enhancement of adenylate cyclase activity in S49 lymphoma. cells by phorbol esters. Putative effect of C kinase on S-GTP catalytic subunit interaction. Journal of Biological Chemistry, 260, 2625–2628.

    Google Scholar 

  3. Bennett, D. C., Dexter, T. J., Ormerod, E. J., and Hart, R., 1986, Increased experimental metastatic capacity of a murine melanoma following induction of differentiation. Cancer Research, 46, 3239–3244.

    Google Scholar 

  4. Bitensky, M. W., Demopoulos, N. B., and Russel, V., 1972, MSH-responsive adenylate cyclase in the Cloudman S91 melanoma. Pigmentation, edited by V. Riley (Des Moines: Meridith Press), pp. 247–255.

    Google Scholar 

  5. Bosmann, H. G., Bieber, G. R., Brown, A. E., Case, K. R., Gersten, D., Kimmerer, T. W., and Lions, A., 1973, Biochemical parameters correlated with tumour cell implantation. Nature, 246, 487–489.

    Google Scholar 

  6. Burchill, S. A., Thody, A. J., and Ito, S., 1986, Melanocyte stimulating hormone, tyrosinase activity and the regulation of eumelanogenesis in the hair follicle melanocytes of the mouse. Journal of Endocrinology, 109, 15–21.

    Google Scholar 

  7. Fuller, B. B., and Viskochil, D. M., 1979, The role of RNA and protein synthesis in mediating the action of MSH on mouse melanoma cells. Life Sciences, 24, 2405–2416.

    Google Scholar 

  8. Gopalakrishna, R., and Barsky, S. H., 1988, Tumour promoter-induced membrane bound protein kinase C regulates hematogenous metastasis. Proceedings of the National Academy of Sciences, U.S.A., 85, 612–616.

    Google Scholar 

  9. Gottesman, M. M., Roth, C., Vlahakis, C. T., and Pastan, I., 1984, Cholera-toxin treatment stimulates tumorgenicity of Rous sarcoma virus-transformed cells. Molecular Cell Biology, 4, 2639–2642.

    Google Scholar 

  10. Gottesman, M. M., and Fleischmann, R. D., 1986, The role of cyclic AMP in regulation of tumour cell growth in culture. Journal of Cell Biology, 97, 480–488.

    Google Scholar 

  11. Halaban, R., Pomerantz, S. H., Marshall, S., Lambert, D. T., and Lerner, A. B., 1983, Regulation of tyrosinase in human melanocytes grown in culture. Journal of Cell Biology, 97, 480–488.

    Google Scholar 

  12. Heyworth, C. M., Whetton, A. D., Kinsella, A. R., and Houslay, M. D., 1984, The phorbol ester TPA inhibits glucagon-stimulated adenylate cyclase activity. FEBS Letters, 170, 38–42.

    Google Scholar 

  13. Hill, S. E., Rees, R. C., and MacNeil, S., 1990, A positive association between agonist-induced cyclic AMP production in vitro and metastatic potential in murine B16 melanoma and hamster fibrosarcoma. Clinical and Experimental Metastasis, 8, 461–474.

    Google Scholar 

  14. Hill, S. E., Bleehen, S. S., and MacNeil, S., 1989, 1,25,dihydroxy-vitamin D3 increases intracellular free calcium in murine B16 melanoma. British Journal of Dermatology, 120, 21–30.

    Google Scholar 

  15. Lester, B. R., Greig, R. G., Buscarino, C., Sheppard, J. R., Corwin, S. P., and Posts, G., 1986, Cyclic AMP metabolism in B16 melanoma clone during the formation of experimental and spontaneous metastases. International Journal of Cancer, 38, 405–411.

    Google Scholar 

  16. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951, Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.

    Google Scholar 

  17. Mac Neil, S., Crawford, A., Amirrasooli, H., Johnson, S., Pollock, A., Ollis, C. A., and Tomlinson, S., 1980, Stimulation of hormone responsive adenylate cyclase activity by a factor present in the cell cytosol. Biochemical Journal, 188(2), 393–400.

    Google Scholar 

  18. Mac Neil, S., Walker, S. W., Senior, H. J., Pollock, A., Brown, B. L., Bleehen, S. S., Munro, D. S., and Tomlinson, S., 1984, Calmodulin activation of adenylate cyclase in the mouse B16 melanoma. Journal of Biochemistry, 224, 453–460.

    Google Scholar 

  19. Niles, R. M., and Makarski, J. S., 1978, Control of melanogenesis in mouse melanoma cells of varying metastatic potential. Journal of the National Cancer Institute, 61, 523–526.

    Google Scholar 

  20. Niles, R. M., and Makarski, J. S., 1978, Hormonal activation of adenylate cyclase in mouse melanoma metastatic variants. Journal of Cell Physiology, 96, 355–360.

    Google Scholar 

  21. Niles, R. M., and Logue, M. P., 1979, Isolation and characterisation of a variant of B16 mouse melanoma resistant to MSH growth inhibition. Journal of Supramolecular Structure, II, 251–258.

    Google Scholar 

  22. Ormerod, E. J., and Hart, I. R., 1989, Different growth responses to agents which elevate cyclic AMP in human melanoma cell lines of high and low experimental metastatic capacity. Clinical and Experimental Metastasis, 7, 85–95.

    Google Scholar 

  23. Pawelek, J., Wong, G., Sansone, M., and Morowitz, J., 1973, Molecular controls in mammalian pigmentation. Yale Journal of Biological Medicine, 46, 430–443.

    Google Scholar 

  24. Raz, A., Zöller, M., and Ben Ze-ev, A, 1986, Cell configuration and adhesive properties of metastasising and non-metastasising B5p73 rat adenocarcinoma cells. Experimental Cell Research, 162, 127–141.

    Google Scholar 

  25. Sheppard, J. R., Lester, B., Doll, J., Buscarino, C., Gonzales, E., Corwin, S., Greig, R., and Poste, G., 1986, Biochemical regulation of adenylate cyclase in murine B16 melanoma clones with different metastatic properties. International Journal of Cancer, 37, 713–722.

    Google Scholar 

  26. Whitehead, R. J., Taylor, D. J., Evanson, J. M., Hart, I. R., and Woolley, D. E., 1988, Demonstration of H2 histamine receptors on human melanoma cells. Biochemical and Biophysical Research Communications, 151, 518–523.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K.

    S. E. Hill & S. MacNeil

  2. Department of Experimental and Clinical Microbiology, Section of Tumour Biology and Immunology, The Medical School, Beech Hill Road, S10 2RX, Sheffield, U.K.

    R. C. Rees

  3. Institute for Cancer Studies, Sheffield University, Sheffield, UK

    S. E. Hill, R. C. Rees & S. MacNeil

Authors
  1. S. E. Hill
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  2. R. C. Rees
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  3. S. MacNeil
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Hill, S.E., Rees, R.C. & MacNeil, S. The regulation of cyclic AMP production and the role of cyclic AMP in B16 melanoma cells of differing metastatic potential. Clin Exp Metast 8, 475–489 (1990). https://doi.org/10.1007/BF00058157

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  • DOI: https://doi.org/10.1007/BF00058157

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