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A positive association between agonist-induced cyclic AMP production in vitro and metastatic potential in murine B16 melanoma and hamster fibrosarcoma

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A positive association between agonist-stimulated cyclic AMP production in vitro and both experimentally induced (B16 melanoma) and spontaneous (fibrosarcoma) metastases were found. Five B16 melanoma cell lines producing varying degrees of lung colonization following intravenous injection and three hamster fibrosarcoma cell lines producing a varying number of metastases in lungs and regional lymph nodes after removal of the primary tumour were studied. Agonist-stimulated (forskolin and melanocyte-stimulating hormone), but not basal cyclic AMP accumulation, increased with increasing metastatic potential. This relationship did not extend to other intracellular signalling systems as determined by investigation of basal or foetal-calf stimulated phosphatidylinositol hydrolysis for either tumour type. Intracellular free calcium was also similar in B16 melanoma cell lines of varying metastatic potential.

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References

  1. Agarwal, K. C., and Parks, R. E., 1983, Forskolin: an antimetastatic agent. International Journal of Cancer, 32, 801–804.

    Google Scholar 

  2. Bennett, D. C., Dexter, T. J., Ormerod, E. J., and Hart, I. R., 1986, Increased experimental metastatic capacity of a murine melanoma following induction of differentiation. Cancer Research, 46, 3239–3244.

    Google Scholar 

  3. Bosmann, H. G., Bieber, G. R., Brown, A. E., Case, K. R., Gersten, D. M., Kimmerer, T. W., and Lione, A., 1973, Biochemical parameters correlated with human cell implantation. Nature, 246, 487–489.

    Google Scholar 

  4. Broek, D., Samiy, N., Fasano, O., Fujiyama, A., Tamonoi, F., Northup, J., and Wigler, M., 1985, Differential activation of yeast adenylate cyclase by wild-type and mutant ras proteins. Cell, 41, 763–769.

    Google Scholar 

  5. Chatterjee, S. K., and Kim, U., 1975, Adenosine, 3′,5′-cyclic monophosphate levels and adenosine 3′5′-monophosphate phosphodiesterase activity in metastasizing and non-metastasizing rat mammary carcinomas. Journal of the National Cancer Institute, 54, 181–186.

    Google Scholar 

  6. Chien, J. L., and Warren, J. R., 1988, Free calcium and calmodulin levels in acinar carcinoma and normal acinar cells of rat pancreas. International Journal of Pancreatology, 3, 113–127.

    Google Scholar 

  7. Gottesman, M. M., Roth, C., Vlahakis, G., and Pastan, I., 1984, Cholera-toxin treatment stimulates tumorgenicity of Rous sarcoma virus-transformed cells. Molecular Cell Biology, 4, 2639–2642.

    Google Scholar 

  8. Gottesman, M. M., and Fleischmann, R. D., 1986, The role of cyclic AMP in regulation of tumour cell growth. Cancer Surveys, 5, 291–308.

    Google Scholar 

  9. Hill, S. E., Bleehen, S. S., and Mac Neil, S., 1989, 1α,25-Dihydroxy vitamin D3 increases intracellular free calcium in murine B16 melanoma. British Journal of Dermatology, 120, 21–30.

    Google Scholar 

  10. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J., 1951, Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.

    Google Scholar 

  11. Mac Neil, S., Walker, S. W., Senior, M. J., Pollock, A., Brown, B. L., Bleehen, S. S., Munro, D. S., and Tomlinson, S., 1984, Calmodulin activation of adenylate cyclase in the mouse B16 melanoma. Journal of Biochemistry, 224, 453–460.

    Google Scholar 

  12. Moolenaar, W. H., Tertoolen, L. G. J., and DeLaat, S. W., 1984, Growth factors immediately raise cytoplasmic free calcium in human fibroblasts. Journal of Biological Chemistry, 259, 8066–8069.

    Google Scholar 

  13. Niles, R. M., and Makarski, J. S., 1978, Hormonal activation of adenylate cyclase in mouse melanoma metastatic variants. Journal of Cellular Physiology, 96, 355–360.

    Google Scholar 

  14. Niles, R. M., and Logue, M. P., 1979, Isolation and characterization of a varient of B16 mouse melanoma resistant to MSH growth inhibition. Journal of Supramolecular Structure, II, 251–258.

    Google Scholar 

  15. Ormerod, E. J., and Hart, I. R., 1989, Different growth responses to agents which elevate cyclic AMP in human melanoma cell lines of high and low experimental metastatic capacity. Clinical and Experimental Metastasis, 7, 85–95.

    Google Scholar 

  16. Padua, R. A., Barrass, N., and Currie, G. A., 1984, A novel transforming gene in a human malignant melanoma cell line. Nature, 311, 671–673.

    Google Scholar 

  17. Sheppard, J. R., Koestler, T. P., Corwin, S. P., Buscarino, C., Dull, J., Lester, B., Greig, R., and Posts, G., 1984, Experimental metastatis correlates with cyclic AMP accumulation in B16 melanoma clones. Nature (London), 308, 544–548.

    Google Scholar 

  18. Sheppard, J. R., Lester, B., Doll, J., Buscarino, C., Gonzales, E., Corwin, S., Greig, R., and Posts, G., 1986, Biochemical regulation of adenylate cyclase in murine B16 melanoma clones with different metastatic properties. International Journal of Cancer, 37, 713–722.

    Google Scholar 

  19. Shoback, D. M., and McGhee, J. M., 1988, Fluoride stimulates the accumulation of inositol phosphates, increases intracellular free calcium and inhibits parathyroid-hormone release in dispersed bovine parathyroid cells. Endocrinology, 122, 2833–2839.

    Google Scholar 

  20. Sugimoto, T., Ritter, C., Ried, I., Morrissey, J., and Slatopolsky, E., 1988, Effect of 1,25 dihydroxy vitamin D3 on cytosolic calcium in dispersed parathyroid cells. Kidney International, 33, 850–854.

    Google Scholar 

  21. Teale, D. M., and Rees, R. C., 1987, Metastatic heterogeneity in a spontaneously metastatic HSV-2 induced hamster fibrosarcoma—association of phenotype and genotype properties with metastatic potential. Invasion and Metastasis, 7, 129–143.

    Google Scholar 

  22. Thorgeirsson, U. P., Turpeenniemi-Hujanen, T., Williams, J. E., Westin, E. H., Heilman, C. A., Talmadge, J. E., and Liotta, L. A., 1985, NIH/3T3 cells transfected with a human tumour DNA containing activated ras oncogenes express the metastatic phenotype in nude mice. Molecular Cell Biology, 5, 259–262.

    Google Scholar 

  23. Vousden, K. H., Eccles, S. A., Purvies, H., and Marshall, C. J., 1986, Enhanced spontaneous metastasis of mouse carcinoma cells transfected with an activated C-Ha-ras-1 gene. International Journal of Cancer, 37, 425–433.

    Google Scholar 

  24. Wakelam, M. J. D., Davies, S. A., Houslay, M. D., McKay, I., Marshall, C. J., and Hall, A., 1986, Normal p21 N-ras couples bombesin and other growth factor receptors to inositol phosphate production. Nature, 323, 173–176.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Clinical Sciences Centre, Northern General Hospital, S5 7AU, Sheffield, U.K.

    S. E. Hill

  2. Department of Experimental and Clinical Microbiology, Section of Tumour Biology and Immunology, The Medical School, Beech Hill Road, S10 2RX, Sheffield, U.K.

    R. C. Rees

  3. Institute for Cancer Studies, Sheffield University, Sheffield, UK

    S. MacNeil

Authors
  1. S. E. Hill
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  2. R. C. Rees
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  3. S. MacNeil
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Hill, S.E., Rees, R.C. & MacNeil, S. A positive association between agonist-induced cyclic AMP production in vitro and metastatic potential in murine B16 melanoma and hamster fibrosarcoma. Clin Exp Metast 8, 461–474 (1990). https://doi.org/10.1007/BF00058156

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  • DOI: https://doi.org/10.1007/BF00058156

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