Abstract
During the first 8–12 days of cultivation in a nutrient solution containing IAA, inositol and kinetin freshly isolated carrot root explants develop into a chlorophyllous and photosynthetically active tissue culture. Electron microscopy, low temperature pigment absorption spectra and fluorescence induction profile recording as well as the determination of the activity of some enzymes (ribulosebisphosphate carboxylase, phosphoenolpyruvate carboxylase) and CO2-fixation experiments were carried out. Based on the results, a sequence of developmental stages of the photosynthetic system will be proposed. During the autotropic period from about the 20th day of culture onward the light reaction system of these tissue cultures is quite comparable to that of carrot leaves; however, some differences in the CO2-fixation mechanism were observed.
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Baldry CW, Bucke C, Coombs T (1970) Effect of some phenoloxidase inhibitors on chloroplasts and carboxylation enzymes of sugar cane and spinach. Planta 94:124–133
Bender L, Kumar A, Neumann KH (1981) Photoautotrophe pflanzliche Gewebekulturen in Laborfermentern. In: Lafferty RM (ed) Fermentation. Springer Verlag, Vienna, New York, pp 193–203
Breidenbach W, Kahn A, Beavers H (1968) Characterization of glyoxysomes from castor bean endosperms. Plant Physiol 43:704–713
Frosch S, Bergfeld R, Mohr H (1976) Light control of plastogenesis and ribulosebi sphosphate carboxylase levels in mustard seedling cotyledons. Planta 133:53–56
Hanson AP, Edelman J (1972) Photosynthesis by carrot tissue cultures. Planta 102:11–25
Kumar A, Bender L, Neumann KH (1980) Characterization of the photosynthetic system of Daucus carota callus cultures. In: Rao PS (ed) Plant Tissue Culture; Genetic Manipulation and Somatic Hybridization of Plant Cells, Bombay, pp 169–178
Kumar A, Bender L, Neumann KH (1983) Growth regulation, plastid differentation and the development of a photosynthetic system in cultured carrot root explants as influenced by exogenous sucrose and various phytohormones. Plant Cell Tissue and Organ Culture: (in press)
Laetsch WM, Stetler DA (1965) Chloroplast structure and function in cultured tobacco tissue. Am J Bot 52:798–804
Luft HJ (1961) Improvements in epoxyresin embedding methods. J Biophys Biochem Cytol 9:409–414
Neumann KH (1962) Untersuchungen über den Einfluß essentieller Schwermetalle auf das Wachstum und den Proteinstoffwechsel von Karottengewebekulturen. Dissertation, Justus-Liebig-Universität Giessen
Neumann KH (1968) Über Beziehungen zwischen hormonal gesteuerter Zellteilungsgeschwindigkeit und Differenzierung. Ein Beitrag zur Physiologie der pflanzlichen Ertragsbildung. Habilitationsschrift, Justus-Liebig-Universität Giessen
Neumann KH, Cireli E, Cireli B (1969) Untersuchungen über Beziehungen zwischen Zellteilung und Morphogenese bei Gewebekulturen von Daucus carota. III. Einfluß des Kinetins auf die Ultrastruktur der Zellen. Physiol Plant 22:787–800
Neumann KH, Raafat A (1973) Further studies on the photosynthesis of carrot tissue cultures. Plant Physiol 51:685–690
Neumann KH, Pertzsch CH, Moos M, Krömmelbein G (1978) Untersuchungen zur Charakterisierung des Ernährungssystems von Karottengewebekulturen: Photosynthese, Kohlenstoff- und Stickstoffernährung, Mineralstoffverbrauch. Z Pflanzenernaehr Bodenk 141:299–311
Neumann KH, Bender L, Kumar A, Szegoe M (1982) Photosynthesis and Pathways of Carbon in Tissue Cultures of Daucus and Arachis. Proc. 5th Intl. Cong. Plant Tissue & Cell Culture, Tokyo, ‘Plant Tissue Culture’, pp 251–252
Pertzsch CH (1976) Untersuchungen zur Photosynthese von Karottengewebekulturen und Karottenbalättern unter Verwendung der Radiogaschromatographie. Ph D thesis, Justus-Liebig-Universität Giessen
Reynolds GS (1963) The use of lead citrate at high pH as an electronopaque stain in electronmicroscopy. J Cell Biol 17:208–212
Senger H, Bornman CH (1976) Welwitschia mirabilis: formation and distribution of chlorophylls. Z Pflanzenphysiol 80:261–270
Stetler DA, Laetsch WM (1969) Chloroplast development in Nicotiana tabaccum ‘Maryland Mammoth’. Am J Bot 56:260–270
Steward FC, Caplin SM, Millar FK (1952) Investigations on the growth and metabolism of plant cells. I. New techniques for the investigation on metabolism, nutrition and growth in undifferentiated cells. Ann Bot (London) 16:57–77
Thomson WW, Whatley JM (1980) Development of nongreen plastids. Ann Rev. Plant Physiol 31:375–394
Wintermans JFGM, De Mots A (1965) Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol. Biochem Acta 109:448–453
Yamada Y, Sato F, Hagimori M (1978) Photoautotrophism in green cultured cells. In: Thorpe TA (ed) Frontiers of Plant Tissue Culture, Calgary, pp 453–461
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Kumar, A., Bender, L., Pauler, B. et al. Ultrastructural and biochemical development of the photosynthetic apparatus during callus induction in carrot root explants. Plant Cell Tiss Organ Cult 2, 161–177 (1983). https://doi.org/10.1007/BF00043361
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DOI: https://doi.org/10.1007/BF00043361