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Synthesis of 5S rRNA and putative precursor tRNAs in nuclei isolated from wheat embryos

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Summary

Nuclei isolated from wheat embryos synthesize 4.5S precursor tRNAs and 5S rRNA in vitro. The precursor tRNAs can be processed to 4S tRNAs. Transcription of the tRNA and 5S rRNA genes is carried out by endogenous RNA polymerase III, and addition of exogenous wheat RNA polymerase III results in increased transcription on these genes. A number of experimental results suggest that proper initiation and accurate transcription of the tRNA and 5S rRNA genes occurs with isolated wheat nuclei.

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Authors and Affiliations

  1. Department of Botany, University of Minnesota, 220 Biological Sciences, 1445 Gortner Avenue, 55108, MN, St. Paul, USA

    Tom J. Guilfoyle, JoAnn Suzich & Margaret Lindberg

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  1. Tom J. Guilfoyle
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  2. JoAnn Suzich
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  3. Margaret Lindberg
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Guilfoyle, T.J., Suzich, J. & Lindberg, M. Synthesis of 5S rRNA and putative precursor tRNAs in nuclei isolated from wheat embryos. Plant Mol Biol 7, 95–104 (1986). https://doi.org/10.1007/BF00040136

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  • DOI: https://doi.org/10.1007/BF00040136

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