Abstract
Extracts from Chlamydomonas, corn, soybean and tobacco were tested for enzymes of the lysine biosynthetic pathway. Dihydrodipicolinic acid (DHD) synthase, DHD reductase, diaminopimelate (DAP) epimerase and DAP decarboxylase were present in all. However, in contrast to the report of Wenko et al., meso-DAP dehydrogenase could not be detected in extracts prepared from soybean. Moreover, it was not found in Chlamydomonas, corn and tobacco as well. In order to set an upper limit to the amount of meso-DAP dehydrogenase that might be present, reconstruction experiments were performed with soybean and corn extracts in which the conversion of dihydrodipicolinate to lysine was made dependent on the addition of limited amounts of the meso-DAP dehydrogenase purified from Bacillus sphaericus. The presence of DAP epimerase and the absence of meso-DAP dehydrogenase indicates that the meso-DAP dehydrogenase abbreviated pathway for lysine synthesis is not operative in plants.
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References
Anderson P, Hibberd K: Evidence for the interaction of an imidazolinone herbicide with leucine, valine and isoleucine metabolism. Weed Sci 33: 479–483 (1985).
Antia M, Hoare DS, Work E: The stereoisomers of α,ε-diaminopimelic acid. Properties and distribution of diaminopimelic acid racemase an enzyme causing interconversion of the LL and meso isomers. Biochem J 65: 448–459 (1957).
Black S, Wright NG: Aspartic β-semialdehyde dehydrogenase and aspartic β-semialdehyde. J Biol Chem 213: 39–50 (1955).
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).
Farkas W, Gilvarg C: The reduction step in diaminopimelic acid biosynthesis. J Biol Chem 240: 4717–4722 (1965).
Frisch DA, Gegenbach BG, Tommey AM, Sellner JM, Somers DA, Myers DE: Isolation and characterization of dihydrodipicolinate synthase from maize. Plant Physiol 96: 444–452 (1991).
Gilvarg C: The enzymatic synthesis of diaminopimelic acid. J Biol Chem 233: 1501–1504 (1958).
Gilvarg C: N-Succinyl-L-diaminopimelic acid. J Biol Chem 234: 2955–2959 (1959).
Kindler SH, Gilvarg C: N-Succinyl-L-α,ε diaminopimelic deacylase. J Biol Chem 235: 3532–3535 (1960).
Margulis L: Mitochondria: acquisition by Whom? Symbiosis in Cell Evolution. W.H. Freeman, San Francisco (1981).
Mathews BF, Widholm JM: Enzyme expression in soybean cotyledons, callus and cell suspension culture. Can J Bot 57: 299–304 (1979).
Mazelis M, Miflin BJ, Pratt HM: A chloroplast localized diaminopimelate decarboxylase in higher plants. FEBS Lett 64: 197–200 (1976).
Mazelis M, Whatley FR, Whatley J: The enzymology of lysine biosynthesis in higher plants. The occurrence, characterization and some regulatory properties of dihydrodipicolinate synthase. FEBS Lett 84: 236–240 (1977).
Millet J, Aubert JP: Le metabolisme de l'acide glutamique au ours de la sporulation chez Bacillus megaterium. Ann Inst Pasteur 98: 282–290 (1960).
Misono H, Togawa H, Yamamoto T, Soda K: Occurrence of m-α-ε diaminopimelate dehydrogenase in Bacillus sphaericus. Biochem Biophys Res Comm 89–93 (1976).
Misono H, Togawa H, Yamamoto T, Soda K: m-α-ε-Diaminopimelate dehydrogenase: distribution and the reaction product. J Bact 137: 22–27 (1979).
Misono H, Soda K: Properties of meso α-ε-DAP dehydrogenase from Bacillus sphaericus. J Biol Chem 255: 10599–10605 (1980).
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Peterkofsky B, Gilvarg C: N-Succinyl-L-diaminopimelate-glutamic transaminase. J Biol Chem 236: 1432–1438 (1961).
Simms SA, Voige WH, Gilvarg C: Purification and characterization of succinyl CoA: tetrahydrodipicolinate succinyl transferase from Escherichia coli. J Biol Chem 259: 2734–2741 (1984).
Sueoka N: Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 46: 83–91 (1960).
Tyagi VVS, Henke RR, Farkas WR: Occurrence of diaminopimelic epimerase in maize. Biochim Biophys Acta 719: 363–369 (1982).
Tyagi VVS, Henke RR, Farkas W: Partial purification and characterization of dihydrodipicolinic acid reductase from maize. Plant Physiol 73: 687–691 (1983).
Warburg O, Christian W: Isolierung und Kristallisation des Gärungsferments Enolase. Biochem Z 310: 384–423 (1941).
Wenko LK, Treick WR, Wilson KG: Isolation and characterization of a gene encoding m-Diaminopimelate dehydrogenase from Glycine max. Plant Mol Biol 4: 197–204 (1985).
White PJ: Diaminopimelate decarboxylase (Escherichia coli). Meth Enzymol 17b: 140–145 (1971).
White PJ: The essential role of diaminopimelate dehydrogenase in the biosynthesis of lysine by Bacillus sphaericus. J Gen Microbiol 129: 739–749 (1983).
Yugari Y, Gilvarg C: The condensation step in diaminopimelate synthesis. J Biol Chem 240: 4710–4716 (1965).
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Chatterjee, S.P., Singh, B.K. & Gilvarg, C. Biosynthesis of lysine in plants: the putative role of meso-diaminopimelate dehydrogenase. Plant Mol Biol 26, 285–290 (1994). https://doi.org/10.1007/BF00039539
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DOI: https://doi.org/10.1007/BF00039539