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Regulatable endogenous production of cytokinins up to ‘toxic’ levels in transgenic plants and plant tissues

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Abstract

The effects of expressing a chimeric gene consisting of a soybean heat shock gene promoter and a sequence that encodes an enzyme catalyzing the synthesis of a potent phytohormone, the cytokinin iPMP, have been analyzed in transgenic tobacco plants. The production of cytokinin endogenously produced several effects previously undocumented. The differentiation of shoots independent of exogenous cytokinin from heat-treated transgenic plant leaf explants demonstrates that long-term heat treatments do not interfere with complex developmental processes. This extends the potential usefulness of heat shock gene promoters to conditionally express genes during windows of development that span several weeks.

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Authors and Affiliations

  1. Botany Department, University of Georgia, 30602, Athens, GA, USA

    W. Michael Ainley, Joscelyn W. Hill, Wilma L. Lingle, Ronald T. Nagao & Joe L. Key

  2. Horticultural Science Department, University of Minnesota, 55108, St. Paul, MN, USA

    Kenneth J. McNeil & Mark L. Brenner

  3. Plant Cell Research Institute, 94568, Dublin, CA, USA

    Robert B. Simpson

Authors
  1. W. Michael Ainley
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  2. Kenneth J. McNeil
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  3. Joscelyn W. Hill
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  4. Wilma L. Lingle
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  5. Robert B. Simpson
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  6. Mark L. Brenner
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  7. Ronald T. Nagao
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  8. Joe L. Key
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Ainley, W.M., McNeil, K.J., Hill, J.W. et al. Regulatable endogenous production of cytokinins up to ‘toxic’ levels in transgenic plants and plant tissues. Plant Mol Biol 22, 13–23 (1993). https://doi.org/10.1007/BF00038992

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  • DOI: https://doi.org/10.1007/BF00038992

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