Abstract
The partial nucleotide sequence of the 3′-terminal region of the Korean isolate of odontoglossum ringspot tobamovirus (ORSV-Cy) from cool-growing Cymbidium was determined. The sequence contained a full length open reading frame (ORF) coding for the viral cell-to-cell movement protein (MP). The ORF was located upstream of the coat protein gene and 105 nucleotides longer than that of tobacco mosaic virus (TMV). The ORF predicts a polypeptide chain of 303 amino acids with a molecular weight of 33573. The ORF contained a similar region of conserved sequence motif of tobamoviruses and putative assembly origin of the viral RNA was located at about 1,100 nucleotides away from the 3′ end. The predicted amino acid sequence for the MP gene of ORSV-Cy is more closely related to pepper mild mottle virus (PMMV), TMV-vulgare and TMV-Rakkyo than to tobacco mild green mosaic virus (TMGMV), TMV-L, cowpea strain of TMV (SHMV), and cucumber green mottle mosaic virus (CGMMV).
References
Alonso, E, Garcia-Luque, I, Cruz, A, Wicke, B, Avila-Rincon, MJ, Serra, MT, Castresana, C, Diaz-Ruiz, JR: Nucleotide sequence of the genomic RNA pepper mild mottle virus, a resistance-breaking tobamovirus in pepper. J Gen Virol 72: 2875–2884 (1991).
Dubs, MC, VanRegenmortel, MHV: Odontoglossum ringspot virus coat protein: sequence and antigenic comparisons with other tobamoviruses. Arch Virol 115: 239–249 (1990).
Goelet, P, Lomonossoff, GP, Butler, PJG, Akam, ME, Gait, MJ, Karn, J: Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79: 5818–5822 (1982).
Isomura, Y, Matumoto, Y, Murayama, A, Chatani, M, Inouye, N, Ikegami, M: Nucleotide sequence of cell-to-cell transport protein gene of odontoglossum ringspot virus. Nucl Acids Res 18: 7448 (1990).
Kwon, SB, Sako, N, Ohshima, K: Nucleotide sequence of the coat protein and 30K protein genes of tobacco mosaic virus-Rakkyo strain locally infecting Nicotiana tabacum L. cv. Bright Yellow. Ann Phytopath Soc Japan 60: 186–195 (1994).
Meshi, T, Ohno, T, Okada, Y: Nucleotide sequence of the 30K protein cistron of cowpea strain of tobacco mosaic virus. Nucl Acids Res 10: 6111–6117 (1982).
Mushegian, AR, Koonin, EV: Cell-to-cell movement of plant viruses. Arch Virol 133: 239–257 (1993).
Ohno, T, Aoyagi, M, Yamanashi, Y, Saito, H, Ikawa, S, Meshi, T, Okada, Y: Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. J Biochem 96: 1915–1923 (1984).
Park, WM, Kim, WG, Ryu, KH, Yoon, KE, Kwack, BH, So, IS: Detection of odontoglossum ringspot virus and cymbidium mosaic virus from cultivated orchids by immunosorbent electron microscopy. J Kor Soc Hort Sci 31: 417–422 (1990).
Park, WM, Yoon, KE, Chung, SY, Ryu, KH: Identification of odontoglossum ringspot virus isolated from Cymbidium goeringii Reichenbach in Korea. Kor J Plant Pathol 6: 387–392 (1990).
Park, WM, Yoon, KE, Chung, SY, Ryu, KH: Purification and serological detection of odontoglossum ringspot virus isolated from Cymbidium goeringii in Korea. Kor J Plant Pathol 6: 474–481 (1990).
Paul HL: Odontoglossum ringspot virus. CMI/AAB Description of Plant Viruses No. 155 (1975).
Ryu, KH, Choi, CW, Park, WM: Molecular cloning and sequence analysis of the 3′-terminal region encoding coat protein of odontoglossum ringspot virus from Cymbidium sp. in Korea. Kor J Plant Pathol Newsletter 5 (1): 91–92 (1994).
Sambrook, J, Fritsch, EF, Maniatis, T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sippel, AE: Purification and characterization of adenosine triphosphateribonucleic acid adenyltransferase from Escherichia coli. Eur J Biochem 37: 31–40 (1973).
Solis, I, Garcia-Arenal, F. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553–558 (1990).
Ugaki, M, Tomiyama, M, Kakutani, T, Hidaka, S, Kiguchi, T, Nagata, R, Sato, T, Motoyoshi, F, Nishiguchi, M: The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA. J Gen Virol 72: 1487–1495 (1991).
Zettler, FW, Ko, NJ, Wisler, GC, Elliott, MS, Wong, SM: Viruses of orchids and their control. Plant Disease 74: 621–626 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ryu, K.H., Park, W.M. Nucleotide sequence analysis of a cDNA clone encoding the 34K movement protein gene of odontoglossum ringspot virus, ORSV-Cy, the Korean isolate. Plant Mol Biol 26, 995–999 (1994). https://doi.org/10.1007/BF00028866
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028866