Abstract
In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putative protein products of 8066 Da and 12849 Da, respectively, each with a signal sequence at the N-terminus followed by a repetitive proline-rich region. Based on their expression during the initial period of nodule development, MsEnod12A and B are alfalfa early nodulin genes.
References
Deak M, Kiss GB, Koncz C, Dudits D: Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep 5: 97–100 (1986).
Dehio C, deBruijn FJ: The early nodulin gene SrEnod2 from Sesbania rostrata is inducible by cytokinin. Plant J 2: 117–128 (1992).
Dickstein R, Bisseling T, Reinhold VN, Ausubel FM: Expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development. Genes Devel 2: 677–687 (1988).
Franssen HJ, Nap J-P, Gloudemans T, Stiekema W, vanDam H, Govers F, Louwerse J, vanKammen A, Bisseling T: Characterization of cDNA for nodulin-75 of soybean: A gene product involved in early stages of root nodule development. Proc Natl Acad Sci USA 84: 4495–4499 (1987).
Gibson AH: Methods for legumes in glasshouses and controlled environment cabinets. In: Bergersen FJ (ed) Methods for Evaluating Biological Nitrogen Fixation, pp. 139–184. Wiley, London (1980).
Govers F, Harmsen H, Michielsen P, Prins M, vanKammen A, Bisseling T: Characterization of the pea ENOD12B gene and expression analyses of the two ENOD12 genes in nodule, stem and flower tissue. Mol Gen Genet 228: 160–166 (1991).
Govers F, Nap J-P, vanKammen A, Bisseling T: Nodulins in the developing root nodule. Plant Physiol Biochem 25: 309–322 (1987).
Györgyey J, Gartner A, Németh K, Magyar Z, Hirt H, Heberle-Bors E, Dudits D: Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. Plant Mol Biol 16: 999–1007 (1991).
Kondorosi E, Banfalvi Z, Kondorosi A: Physical and genetic analysis of a symbiotic region of R. meliloti: identification of nodulation genes. Mol Gen Genet 193: 445–452 (1984).
Lerouge P, Roche P, Faucher C, Maillet F, Truchet G, Promé JC, Dénarié J: Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal. Nature 344: 781–784 (1990).
Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Nap J-P, Bisseling T: Developmental biology of a plant-prokaryote symbiosis: The legume root nodule. Science 250: 948–954 (1990).
Pay A, Heberle-Bors E, Hirt H: An alfalfa cDNA encodes a protein with homology to translationally controlled human tumor protein. Plant Mol Biol 19: 501–503 (1992).
Scheres B, van deWiel C, Zalensky A, Horvath B, Spaink H, vanEck H, Zwartkruis J, Wolters A-M, Gloudemans T, vanKammen A, Bisseling T: The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction. Cell 60: 281–294 (1990).
Scheres B, vanEngelen F, van derKnaap E, van deWiel C, vanKammen A, Bisseling T: Sequential induction of nodulin gene expression in the developing pea nodule. Plant Cell 2: 687–700 (1990).
Trese AT, Pueppke SG: Cloning of cowpea (Vigna unguiculata) genes that are regulated during initiation of nodulation. Mol Plant-Microb Inter 4: 46–51 (1991).
van deWiel C, Scheres B, Franssen H, vanLierop M-J, vanLammeren A, vanKammen A, Bisseling T: The early nodulin transcript ENOD2 is located in the nodule parenchyma (inner cortex) of pea and soybean root nodules. EMBO J 9: 1–7 (1990).
Vincze E, Kiss GB: A phosphate group at the cos ends of phage lambda DNA is not a prerequisite for in vitro packaging; an alternative method for constructing genomic libraries using a new plasmid vector, pGY97. Gene 96: 17–22 (1990).
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Allison, L.A., Kiss, G.B., Bauer, P. et al. Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family. Plant Mol Biol 21, 375–380 (1993). https://doi.org/10.1007/BF00019952
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DOI: https://doi.org/10.1007/BF00019952