Abstract
Two cDNA clones encoding two different β-tubulins, RTUB-1 and RTUB-2, were isolated from a rice cDNA library and their nucleotide sequences were analyzed. The deduced amino acid sequences showed amino acid sequence identity between 92% and 97% with other plant β-tubulins. Southern blot analysis using gene-specific and coding-region probes suggested that β-tubulins in rice are encoded by multigene families. The two cDNA clones represent two subfamilies of rice tubulins. RTUB-1 and RTUB-2, consisting of 3 to 4 genes and a single gene, respectively. The transcript levels of RTUB-1 and RTUB-2 genes were higher in actively elongating tissues such as etiolated shoot tissues and light-grown root tissues of four-day old seedlings.
This is a preview of subscription content, log in via an institution to check access.
References
Bustos MM, Guiltinan MJ, Cyr RJ, Ahdoot D, Fosket DE: Light regulation of β-tubulin gene expression during internode development in soybean (Glycine max [L.] Merr.). Plant Physiol 91: 1157–1161 (1989).
Carpenter JL, Ploense SE, Snustad DP, Silflow CD: Preferential expression of an α-tubulin gene of Arabidopsis in pollen. Plant Cell 4: 557–571 (1992).
Carpenter JL, Kopczak SD, Snustad DP, Silflow CD: Semi-constitutive expression of an Arabidopsis thaliana α-tubulin gene. Plant Mol Biol 21: 937–942 (1993).
Cleveland DW: The multitubulin hypothesis revisited: what have we learned? J Cell Biol 104: 381–383 (1987).
Cleveland DW, Sullivan KF: Molecular biology and genetics of tubulin. Annu Rev Biochem 54: 331–365 (1985).
Colbert JT, Costigan SA, Zhao Z: Photoregulation of β-tubulin mRNA abundance in etiolated oat and barley seedlings. Plant Physiol 93: 1196–1202 (1990).
Cowan NJ, Lewis SA, Gu W, Burgoyne RD: Tubulin isotypes and their interaction with microtubule associated proteins. Protoplasma 145: 106–111 (1988).
Fosket DE, Morejohn LC: Structural and functional organization of tubulin. Annu Rev Plant Physiol Plant Mol Biol 43: 201–240 (1992).
Guiltinan MJ, Ma DP, Barker RF, Bustos MM, Cyr RJ, Yadegari R, Fosket DE: The isolation, characterization and sequence of two divergent β-tubulin genes from soybean (Glycine max L.). Plant Mol Biol 10: 171–184 (1987).
Han IS, Jongewaard I, Fosket DE: Limited expression of a diverged β-tubulin gene during soybean (Glycine max [L.] Merr.) development. Plant Mol Biol 16: 225–234 (1991).
Hepler PK, Palevitz BA: Microtubules and microfilaments. Annu Rev Plant Physiol 24: 309–362 (1974).
Hoyle DH, Raff EC: Two Drosophila beta tubulin isoforms are not functionally equivalent. J Cell Biol 111: 1009–1026 (1990).
Hussey PM, Haas N, Hunsperger J, Larkin J, Snustad DP, Silflow CD: The β-tubulin gene family in Zea mays: two differentially expressed β-tubulin genes. Plant Mol Biol 15: 957–972 (1990).
Hussey PJ, Snustad DP, Silflow CD: Tubulin gene expression in higher plants. In: Lyoyd C (ed). The Cytoskeletal Basis of Plant Growth and Form, pp. 15–28. Academic Press, London (1991).
Kopczak SD, Haas NA, Hussey PJ, Silflow CD, Snustad DP: The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell 4: 539–547 (1992).
Liaud MF, Brinkmann H, Cerff R: The β-tubulin gene family of pea: primary structures, genomic organization and intron-dependent evolution of genes. Plant Mol Biol 18: 639–651 (1992).
Lloyd CW: The plant cytoskeleton: the impact of fluorescence microscopy. Annu Rev Plant Physiol 38: 119–139 (1987).
Marks MD, West J, Weeks DP: The relatively large β-tubulin family of Arabidopsis contains a member with an unusual transcribed 5′ noncoding sequence. Plant Mol Biol 10: 91–104 (1987).
Oppenheimer DG, Haas N, Silflow CD, Snustad DP: The β-tubulin gene family of Arabidopsis thaliana: preferential accumulation of the β1 transcript in roots. Gene 63: 87–102 (1988).
Raff EC: Genetics of microtubule function. J Cell Biol 99: 1–10 (1984).
Rogers HJ, Greenland AJ, Hussey PJ: Four members of the maize β-tubulin gene family are expressed in the male gametophyte. Plant J 4: 875–882 (1993).
Silflow CD, Oppenheimer DG, Kopczak SD, Ploense SE, Ludwig SR, Haas N, Snustad DP: Plant tubulin genes: structure and differential expression during development. Devel Genet 8: 435–460 (1987).
Snustad DP, Haas NA, Kopczak SD, Silflow CD: The small genome of Arabidopsis contains at least nine expressed β-tubulin genes. Plant Cell 4: 549–556 (1992).
Sullivan KF, Cleveland DW: Identification of conserved isotype-defining variable region sequences for four vertebrate β-tubulin polypeptide classes. Proc Natl Acad Sci USA 83: 4327–4331 (1986).
Traas J: The cytoskeleton in plant cell growth, differentiation and cell wall synthesis. Ph. D. thesis, University of Nijmegen (1986).
Wiche G, Oberkanins C, Himmler A: Molecular structure and function of microtubule-associated proteins. Int Rev Cytol 124: 217–273 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kang, M.S., Choi, Y.J., Kim, M.C. et al. Isolation and characterization of two β-tubulin cDNA clones from rice. Plant Mol Biol 26, 1975–1979 (1994). https://doi.org/10.1007/BF00019507
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00019507