Skip to main content
SpringerLink
Log in

The unusual 5′ splicing border GC is used in myrosinase genes of the Brassicaceae

  • Short Communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Myrosinase (thioglucosidase glucohydrolase; EC 3.2.3.1) is a group of isoenzymes in the Brassicaceae, which hydrolyze glucosinolates. Genes encoding myrosinase contain 12 exons and 11 introns. Sequence comparison of two myrosinase genes from Arabidopsis thaliana, TGG1 and TGG2, with the corresponding cDNA from leaves, showed preferential use of a GC dinucleotide as the 5′ splicing border in intron 1 instead of an adjacent GT dinucleotide four bp further 3′. This 5′ GC splice site is conserved in all seven myrosinase genes characterized from three different species. Likewise, in the 3′ region of intron 1 two AG dinucleotides are located seven bp apart. Only the most 5′ of these dinucleotides was found to be used in splicing. Sequence analyses of TGG1 cDNA isolated from seeds, siliques and vegetative tissue using reverse transcription PCR showed that the splicing pattern of this intron is identical in these tissues for TGG1. The GT and the most 3′ AG dinucleotides mentioned above have been assumed to be the intron borders of intron 1 in several myrosinase genes. The present investigation shows that this assumption is not correct.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. AminKM, ScarpaAL, WinkelmannJC, CurtisPJ, ForgetBG: The exon-intron organization of the human erythroid β-spectrin gene. Genomics 18: 118–125 (1993).

    Article  PubMed  Google Scholar 

  2. ChadchawanS, BishopJ, ThangstadOP, BonesAM, Mitchell-OldsT, BradleyD: Arabidopsis cDNA sequence encoding myrosinase. Plant Physiol 103: 671–672 (1993).

    Article  PubMed  Google Scholar 

  3. GoodallGJ, FilipowiczW: Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants. EMBO J 10: 2635–2644 (1991).

    PubMed  Google Scholar 

  4. JacksonIJ: A reappraisal of non-consensus mRNA splice sites. Nucl Acids Res 19: 3795–3798 (1991).

    PubMed  Google Scholar 

  5. Kater MM: Structure, function and expression of plant and bacterial enoyl-ACP reductase genes. Thesis, Vrije Universiteit Amsterdam, pp. 61–84 (1994).

  6. LearAL, EperonLP, WheatleyIM, EperonIC: Hierarchy for 5′ splice site preference determined in vivo. J Mol Biol 211: 103–115 (1990).

    PubMed  Google Scholar 

  7. LenmanM, FalkA, XueJ, RaskL: Characterization of a Brassica napus myrosinase pseudogene: myrosinases are members of the BGA family of β-glycosidases. Plant Mol Biol 21: 463–474 (1993).

    Article  PubMed  Google Scholar 

  8. LouH, McCulloughAJ, SchulerMA: 3′ Splice site selection in dicot plant nuclei is position dependent. Mol Cell Biol 13: 4485–4493 (1993).

    PubMed  Google Scholar 

  9. MachlinS, Mitchell-OldsT, BradleyD: Sequence of a Brassica campestris myrosinase gene. Plant Physiol 102: 1359–1360 (1993).

    Article  PubMed  Google Scholar 

  10. McCulloughAJ, LouH, SchulerMA: Factors affecting authentic 5′ splice site selection in plant nuclei. Mol Cell Biol 13: 1323–1331 (1993).

    PubMed  Google Scholar 

  11. MenonKP, TieuPT, NeufeldEF: Architecture of the canine IDUA gene and mutation underlying canine mucopolysaccharidosis I. Genomics 14: 763–768 (1992).

    PubMed  Google Scholar 

  12. NelsonKK, GreenMR: Mechanism for cryptic splice site activation during pre-mRNA splicing. Proc Natl Acad Sci USA 87: 6253–6257 (1990).

    PubMed  Google Scholar 

  13. OritaM, IwahanaH, KanazawaH, HayashiK, SekiyaT: Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 86: 2766–2770 (1989).

    PubMed  Google Scholar 

  14. SchwartzBW, SloanJS, BeckerWM: Characterization of genes encoding hydroxypyruvate reductase in cucumber. Plant Mol Biol 17: 941–947 (1991).

    PubMed  Google Scholar 

  15. ShapiroMB, SenapathyP: RNA splice functions of different classes of eukaryotes: statistics and functional implications in gene expression. Nucl Acids Res 15: 7155–7174 (1987).

    PubMed  Google Scholar 

  16. SpivakJG, WoodsGM, FraserNW: Identification of a novel latency-specific splice donor signal within the herpex simplex virus type 1 2.0 kilobase latency-associated transcript (LAT): translation inhibition of LAT open reading frames by the intron within the 2.0-kilobase LAT. J Virol 65: 6800–6810 (1991).

    PubMed  Google Scholar 

  17. SrivastavaVK, HillDC: Glucosinolates hydrolytic products given by Sinapis alba and Brassica napus thioglucosidase. Phytochemistry 13: 1043–1046 (1974).

    Article  Google Scholar 

  18. ThangstadOP, WingeP, HusebyeH, BonesA: The myrosinase (thioglucoside glucohydrolase) gene family in Brassicaceae. Plant Mol Biol 23: 511–524 (1993).

    PubMed  Google Scholar 

  19. TroelstraC, HesenW, BootsmaD, HoeijmakersJHJ: Structure and expression of the excision repair gene ERCC6 involved in the human disorder Cockayne's syndrome group B. Nucl Acids Res 21: 419–426 (1993).

    PubMed  Google Scholar 

  20. Xue J, Jørgensen M, Pihlgren U, Rask L: The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution. Plant Mol Biol, in press (1995).

  21. XueJ, LenmanM, FalkA, RaskL: The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family. Plant Mol Biol 18: 387–398 (1992).

    Google Scholar 

  22. XueJ, PihlgrenU, RaskL: Temporal, cell-specific, and tissue-preferential expression of myrosinase genes during embryo and seedling development in Sinapis alba. Planta 191: 95–101 (1993).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Jiaping Xue

    Present address: Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden

Authors and Affiliations

  1. Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden

    Lars Rask

  2. Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada

    Jiaping Xue

Authors
  1. Jiaping Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lars Rask
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xue, J., Rask, L. The unusual 5′ splicing border GC is used in myrosinase genes of the Brassicaceae. Plant Mol Biol 29, 167–171 (1995). https://doi.org/10.1007/BF00019128

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00019128

Key words

Search

Navigation