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PTH-C1: a rat continuous cell line expressing the parathyroid phenotype

  • Endocrine Methods and Techniques
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Abstract

The lack of a continuous cell line of epithelial parathyroid cells able to produce parathyroid hormone (PTH) has hampered the studies on in vitro evaluation of the mechanisms involved in the control of parathyroid cell function and proliferation. The PT-r cell line was first established from rat parathyroid tissue in 1987, but these cells were known to express the parathyroid hormone-related peptide (Pthrp) gene, but not the Pth gene. In an attempt to subclone the PT-r cell line, a rat parathyroid cell strain was isolated and named PTH-C1. During 3 years, in culture, PTH-C1 cells maintained an epithelioid morphology, displaying a diploid chromosome number, a doubling time around 15 h during the exponential phase of growth, and parathyroid functional features. PTH-C1 cell line produces PTH and expresses the calcium sensing receptor (Casr) gene and other genes known to be involved in parathyroid function. Most importantly, the PTH-C1 cells also exhibit an in vitro secretory response to calcium. Altogether these findings indicate the uniqueness of the PTH-C1 cell line as an in vitro model for cellular and molecular studies on parathyroid physiopathology.

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Acknowledgments

This work was supported by an unrestricted Grant of F.I.R.M.O, Fondazione Raffaella Becagli (to MLB).

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  1. Department of Surgery and Translational Medicine, University of Florence, Largo Brambilla 3, 50134, Florence, Italy

    Sergio Fabbri, Simone Ciuffi, Valeria Nardone, Ana Rita Gomes, Carmelo Mavilia, Roberto Zonefrati, Gianna Galli, Ettore Luzi, Annalisa Tanini & Maria Luisa Brandi

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  1. Sergio Fabbri
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Correspondence to Maria Luisa Brandi.

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Gerald D. Aurbach, who inspired our work since the very beginning.

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Fabbri, S., Ciuffi, S., Nardone, V. et al. PTH-C1: a rat continuous cell line expressing the parathyroid phenotype. Endocrine 47, 90–99 (2014). https://doi.org/10.1007/s12020-014-0229-7

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