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Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules

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Abstract

Oil bodies (OBs) are specialised organelles ubiquitously detected in plant oil seeds, which serve as lipid storage compartments. OBs consist of a hydrophobic core of triacylglycerol (TAGs), surrounded by a monolayer of phospholipids (PLs) embedded with some specific proteins with a size ranging from 0.5 to 2 μm. In this work, we report an easy method to reconstitute OBs starting from their constituents and to encapsulate lipophilic molecules, i.e. the fluorescent fluorescein isothiocyanate (FITC) and carboxyfluorescein (CF), into reconstituted OBs. This methods allowed us to produce OBs 4- to 10-fold smaller (50–200 nm) than the native one and to obtain a good recovery (about 40%) of both the fluorescent compounds used in the present work. The properties of reconstituted OBs were investigated by a combination of Brewster angle microscopy, scanning force microscopy, ζ-potential techniques. OBs were stable and formed ordered monolayers when patterned on hydrophobic substrates whereas they showed a higher tendency to aggregate into larger, coalescing OBs when were deposited onto hydrophilic substrates or at the air/water interface. Furthermore, we verified the uptake of FITC-loaded OBs by the MCF-7 breast cancer cell line. Our results indicated that OBs could be envisaged as novel carriers to deliver hydrophobic bioactive compounds.

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Acknowledgements

We thank Mr. Luigi Dimo for assistance and performing all Langmuir experiments.

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Authors and Affiliations

  1. Institute of Sciences of Food Production CNR Unit of Lecce, Via Monteroni, 73100, Lecce, Italy

    Stefania Bonsegna, Giovanna Giovinazzo & Angelo Santino

  2. Dipartimento di Ingegneria dell’Innovazione, Università del Salento, 73100, Lecce, Italy

    Simona Bettini, Rosanna Pagano & Ludovico Valli

  3. NNL-Nanoscience Institute of CNR Lecce, IIT Research Unit, Via Arnesano, 73100, Lecce, Italy

    Antonella Zacheo, Viviana Vergaro & Stefano Leporatti

  4. Department of Chemistry and CSGI Consortium, University of Florence, Via della Lastruccia, 3 50019, Sesto Fiorentino, Florence, Italy

    Gabriella Caminati

Authors
  1. Stefania Bonsegna
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  2. Simona Bettini
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  3. Rosanna Pagano
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  4. Antonella Zacheo
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  5. Viviana Vergaro
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  6. Giovanna Giovinazzo
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  7. Gabriella Caminati
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  8. Stefano Leporatti
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  9. Ludovico Valli
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  10. Angelo Santino
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Corresponding author

Correspondence to Angelo Santino.

Additional information

Stefania Bonsegna and Simona Bettini contributed equally to this work.

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Bonsegna, S., Bettini, S., Pagano, R. et al. Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules. Appl Biochem Biotechnol 163, 792–802 (2011). https://doi.org/10.1007/s12010-010-9083-0

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  • DOI: https://doi.org/10.1007/s12010-010-9083-0

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