Abstract
Banana and plantain (Musa spp.) are grown in more than 120 countries in tropical and subtropical regions and constitute an important staple food for millions of people. A Musa acuminata ssp. malaccencis DH Pahang bacterial artificial chromosome (BAC) library (MAMB) was submitted for BAC-end sequencing. MAMB consists of 23,040 clones, with a 140-kbp average insert size, accounting for a five times coverage of the banana genome. A total of 46,080 reads were generated, and 42,750 (92.8%) high-quality sequences were obtained after trimming for vector and quality. Analysis of these data shows a GC content of 41.39%, whereas interspersed repeats comprise 32.3%. The most common repeated sequences found show homology to ribosomal RNA genes, particularly 18S rRNA, while the Ty3/gypsy type monkey retrotransposon is the most common retro element. The sequence data were used to generate a banana-specific repeat library containing 54 new repetitive elements which accounted for 11.86% of the total nucleotides. Simple sequence repeats represent 0.7% of the sequence data and allowed the identification of 2,455 potentially useful marker sites. Functional annotation identified 2,705 sequences that could code for proteins of known function. Microsynteny analysis shows a higher number of co-linear matches to Oryza sativa, in contrast to Arabidopsis thaliana. This database of BAC-end sequences is useful for the assembly of the complete banana genome sequence and is important for identification in functional genomics experiments.
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Acknowledgments
This project was funded through a grant of the Stichting Het Groene Woudt and was partially supported by the Dioraphte Foundation. We thank Dr. Jane Grimwood (HudsonAlpha Institute for Bioinformatics, Huntsville, AL, USA) for the BAC-end sequencing and Stefaan Vandamme and Dr. Kris Laukens (CEPROMA, Antwerp, Belgium) for technical assistance with the protein searching.
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Arango, R.E., Togawa, R.C., Carpentier, S.C. et al. Genome-wide BAC-end sequencing of Musa acuminata DH Pahang reveals further insights into the genome organization of banana. Tree Genetics & Genomes 7, 933–940 (2011). https://doi.org/10.1007/s11295-011-0385-3
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DOI: https://doi.org/10.1007/s11295-011-0385-3