Photosynthetica 2004, 42(4):579-583 | DOI: 10.1007/S11099-005-0016-z

A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants

H. Küpper1,3,*, I. Šetlík2,3, M. Hlásek4
1 Mathematisch-Naturwissenschaftliche Sektion, Fachbereich Biologie, Universität Konstanz, Konstanz, Germany
2 Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
3 Institute of Physical Biology, University of South Bohemia, Nové Hrady, Czech Republic
4 Ing. Milan Hlásek L-tronic, Nádražní 39, Třeboň

A new chamber was developed for a simultaneous measurement of fluorescence kinetics and oxygen exchange in filamentous and thallous algae as well as in small leaves of water plants. Algal filaments or thalli are kept by a stainless grid close to the bottom window of the chamber in the sample compartment. The grid separates the object from the electrode compartment with the oxygen electrode at the top. This compartment accommodates, in addition, a magnetic stirrer that provides efficient circulation of the medium between the sample and the electrode. This magnetic bar spins on a fixed axis and is driven by an electronically commutated magnetic field produced by four coils which are arranged around the chamber. This design yields a very favourable signal to noise ratio in the oxygen electrode records. Consequently, measurements can be performed even of algae with very low photosynthetic rates such as marine low-light red algae or algae under severe stress. For irradiation of the samples and for fluorescence measurements a fibre optic light guide is used facing the window of the chamber. The four branches of a commercially available light guide serve the following purposes: collection of sample fluorescence and supply of measuring, actinic, and saturating light, respectively.

Additional key words: Antithamnion; chlorophyll fluorescence induction; Ectocarpus; gas exchange; photosynthetic oxygen release; photosynthesis; submerged aquatic plants

Received: May 17, 2004; Accepted: September 20, 2004; Published: December 1, 2004  Show citation

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Küpper, H., Šetlík, I., & Hlásek, M. (2004). A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants. Photosynthetica, 42(4), 579-583. doi: 10.1007/S11099-005-0016-z
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    References

    1. Campbell, D., Hurry, V., Clarke, A.K., Gustafsson, P., Öquist, G.: Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation.-Microbiol. mol. Biol. Rev. 62: 667-683, 1998. Go to original source...
    2. Clark, L.C.: Monitor and control of blood and tissue oxygen tensions.-Trans. amer. Soc. art. int. Organs 2: 41-45, 1956.
    3. Dring, M.J., Makarov, V., Schoschina, E., Lorenz, M., Lüning, K.: Influence of ultraviolet-radiation on chlorophyll fluorescence and growth in different life-history stages of three species of Laminaria (Phaeophyta).-Mar. Biol. 126: 183-191, 1996. Go to original source...
    4. Küpper, H., Šetlík, I., Spiller, M., Küpper, F.C., Prášil, O.: Heavy metal-induced inhibition of photosynthesis: targets of in vivo heavy metal chlorophyll formation.-J. Phycol. 38: 429-441, 2002. Go to original source...
    5. Land, S.C., Porterfield, D.M., Sanger, R.H., Smith, P.J.S.: The self-referencing oxygen-selective microelectrode: detection of transmembrane oxygen flux from single cells.-J. exp. Biol. 202: 211-218, 1999. Go to original source...
    6. Maier, I., Calenberg, M.: Effect of extracellular Ca2+ and Ca2+antagonists on the movement and chemoorientation of male gametes of Ectocarpus siliculosus (Phaeophyceae).-Bot. Acta 107: 451-460, 1994. Go to original source...
    7. Mancuso, S., Papeschi, G., Marras, A.M.: A polarographic, oxygen-selective, vibrating-microelectrode system for the spatial and temporal characterisation of transmembrane oxygen fluxes in plants.-Planta 211: 384-389, 2000. Go to original source...
    8. Masojídek, J., Torzillo, G., Kopecký, J., Koblížek, M., Nidiaci, L., Komenda, J., Lukavská A., Sacchi, A.: Changes in chlorophyll fluorescence quenching and pigment composition in the green alga Chlorococcum sp. grown under nitrogen deficiency and salinity stress.-J. appl. Phycol. 12: 417-426, 2000. Go to original source...
    9. Pearson, G., Kautsky, L., Serrão, E.: Recent evolution in Baltic Fucus vesiculosus: reduced tolerance to emersion stresses compared to intertidal (North Sea) populations.-Mar. Ecol. Progr. Ser. 202: 67-79, 2000. Go to original source...
    10. Porterfield, D.M., Smith, P.J.S.: Single-cell, real-time measurements of extracellular oxygen and proton fluxes from Spirogyra grevilleana.-Protoplasma 212: 80-88, 2000. Go to original source...
    11. Yasukawa, T., Kaya, T., Matsue, T.: Characterization and imaging of single cells with scanning electrochemical microscopy.-Electroanalysis 12: 653-659, 2000. Go to original source...

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