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Identification and Characterization of Toxigenic Fusaria Associated with Sorghum Grain Mold Complex in India

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Abstract

Fusarium species are dominant within the sorghum grain mold complex. Some species of Fusarium involved in grain mold complex produce mycotoxins, such as fumonisins. An attempt was made to identify Fusarium spp. associated with grain mold complex in major sorghum-growing areas in India through AFLP-based grouping of the isolates and to further confirm the species by sequencing part of α-Elongation factor gene and comparing the sequences with that available in the NCBI database. The dendrogram generated from the AFLP data clustered the isolates into 5 groups. Five species of Fusarium—F. proliferatum, F. thapsinum, F. equiseti, F. andiyazi and F. sacchari were identified based on sequence similarity of α-Elongation factor gene of the test isolates with those in the NCBI database. Fusarium thapsinum was identified as predominant species in Fusarium—grain mold complex in India and F. proliferatum as highly toxigenic for fumonisins production. Analysis of molecular variance (AMOVA) revealed 54% of the variation in the AFLP patterns of 63 isolates was due to the differences between Fusarium species, and 46% was due to differences between the strains within a species.

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Acknowledgments

The research was supported by The Sehgal Foundation Endowment Fund instituted at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru—502324, AP, India.

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  1. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad, Andhra Pradesh, 502324, India

    Rajan Sharma, Ram P. Thakur, Senapathy Senthilvel, Spurthi Nayak, S. Veera Reddy, Veeranki Panduranga Rao & Rajeev K. Varshney

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  1. Rajan Sharma
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  2. Ram P. Thakur
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  3. Senapathy Senthilvel
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  4. Spurthi Nayak
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  5. S. Veera Reddy
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  6. Veeranki Panduranga Rao
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  7. Rajeev K. Varshney
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Correspondence to Rajan Sharma.

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Sharma, R., Thakur, R.P., Senthilvel, S. et al. Identification and Characterization of Toxigenic Fusaria Associated with Sorghum Grain Mold Complex in India. Mycopathologia 171, 223–230 (2011). https://doi.org/10.1007/s11046-010-9354-x

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  • DOI: https://doi.org/10.1007/s11046-010-9354-x

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