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Xylitol production by Pseudomonas gessardii VXlt-16 from sugarcane bagasse hydrolysate and cost analysis

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Abstract

Xylitol is a well-known sugar alcohol with exponentially rising market demand due to its diverse industrial applications. Organic agro-industrial residues (OAIR) are economic alternative for the cost-effective production of commodity products along with addressing environmental pollution. The present study aimed to design a process for xylitol production from OAIR via microbial fermentation with Pseudomonas gessardii VXlt-16. Parametric analysis with Taguchi orthogonal array approach resulted in a conversion factor of 0.64 g xylitol/g xylose available in untreated sugarcane bagasse hydrolysate (SBH). At bench scale, the product yield increased to 71.98/100 g (0.66 g/L h). 48.49 g of xylitol crystals of high purity (94.56%) were recovered after detoxification with 2% activated carbon. Cost analysis identified downstream operations as one of the cost-intensive parts that can be countered by adsorbent recycling. Spent carbon, regenerated with acetic acid washing can be reused for six cycles effectively and reduced downstream cost by about ≈32%. The strategy would become useful in the cost-effective production of several biomass-dependent products like proteins, enzymes, organic acids, as well.

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Abbreviations

XR:

Xylose reductase

XDH:

Xylitol dehydrogenase

XI:

Xylose isomerase

LCR:

Lignocellulosic residues

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  1. Vishal Ahuja and Arvind Kumar Bhatt contributed equally.

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  1. Department of Biotechnology, Himachal Pradesh University, Shimla, Himachal Pradesh, India

    Vishal Ahuja, Arvind Kumar Bhatt, Sanjeev Mehta, Vaishali Sharma, Ranju Kumari Rathour &  Sheetal

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  1. Vishal Ahuja
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  2. Arvind Kumar Bhatt
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Contributions

VA and AKB: conceptualization, methodology, investigation, and writing—review and editing; SM and S: investigation; RKR and VS: data validation and visualization.

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Correspondence to Vishal Ahuja or Arvind Kumar Bhatt.

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Ahuja, V., Bhatt, A.K., Mehta, S. et al. Xylitol production by Pseudomonas gessardii VXlt-16 from sugarcane bagasse hydrolysate and cost analysis. Bioprocess Biosyst Eng 45, 1019–1031 (2022). https://doi.org/10.1007/s00449-022-02721-z

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